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丙型肝炎病毒NS3蛋白辅助T细胞表位研究 被引量:2

The Study of a Helper T-cell Epitope in Hepatitis C Virus Non-structural 3 Protein
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摘要 对 2例HCV持续性感染者 2个时间点NS3区C末端克隆测序 ,研究HCVNS3蛋白一个辅助T细胞表位 (氨基酸位 12 4 8 12 61)在HCV感染者体内的保守性 ;人工合成该表位进行淋巴细胞增殖试验和抑制试验 ,观察该表位引起免疫应答的水平和特点 ;通过“刺激 休息 刺激”多轮循环建立该表位特异性的T细胞系 ,并用流式细胞仪鉴定表型。结果发现该表位在 2个病人 2个时间点均未变化 ;观察的 2例HCV持续性感染者和 1例感染恢复者均对该表位有较强CD4 +T细胞应答。建立了针对该表位的表型均一的CD4 +辅助T细胞系。本研究提示该表位是一个序列保守的强辅助T细胞表位 ,对HCVT细胞疫苗的研制有一定意义。 The genes of C terminal of HCV NS3 region of two chronic patients in two different time were sequenced to analyse the conservation of a helper T cell epitope in HCV NS3 protein (amino acids 1248 to 1261). The epitope was synthesized and the immune response to it in a self limited patient and two chronic patients was detected using T lymphocyte proliferation assay and inhibition experiment. The epitope specific T cell line was established by several cycles of “stimulation relax stimulation” and analysed with a FACScan. The results indicated that the epitope didn't change in two chronic patients, all three patients had strong CD4 + T cell reponse to the epitope, and epitope specific CD4 + T cell line was established. These data suggest that the eptiope is a conserved strong helper T cell epitope and could become a promising candidate for designing a CD4 + T cell vaccine.
出处 《中国病毒学》 CSCD 2000年第4期318-322,共5页 Virologica Sinica
基金 国家自然科学基金重点资助项目!(396 30 0 2 0 )
关键词 丙型肝炎病毒 辅助T细胞 表位 T细胞系 Hepatitis C virus Helper T cell Epitope T cell l
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参考文献10

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同被引文献48

  • 1赵军,李靖,陈昊,舒翠莉,高蓉,李伯安,何卫平,迟淑萍,程云.HCV高变区1串联模拟表位基因免疫研究[J].第三军医大学学报,2004,26(24):2254-2257. 被引量:8
  • 2史霖,刘珊,胜利,孟照俊,袁育康.丙型肝炎病毒多细胞毒性T淋巴细胞表位DNA疫苗诱导特异性细胞毒性T淋巴细胞反应[J].中华肝脏病杂志,2006,14(5):385-386. 被引量:3
  • 3高军,龚育平,赵平,杨晓平,戚中田.丙型肝炎病毒多表位抗原基因的构建与免疫原性研究[J].中国科学(C辑),2006,36(4):346-354. 被引量:9
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