人骨髓基质细胞的长期培养及其对病毒的敏感性

The Long-term Culture of human bone marrow stromal cells in vitro and their susceptibiility to viruses

为了长期培养人骨髓基质细胞和研究其对病毒的敏感性 ,我们采用静置贴壁培养法 ,体外长期培养了胎儿、儿童和成人骨髓基质细胞 ,并将传至 5代以上的肌样骨髓基质细胞采用微量细胞病变 (CPE)法 ,开展了对 5种病毒的敏感性试验。结果表明 ,人骨髓基质肌样细胞对滤泡性口腔炎病毒 ,脊髓灰质炎病毒、I型和Ⅱ型单纯疱疹病毒均敏感 ,能产生明显的CPE ,其效价 (TCID50 )可达10 - 3～ 10 - 4,其中胎儿骨髓基质肌样细胞对病毒敏感性至少比儿童高 2倍 ,比成人高 4倍 ,即胎儿>儿童 >成人 ,但对B3型柯萨奇病毒均不敏感。我们的研究结果不仅可为人骨髓基质细胞体外长期培养提供了确实可行的简便方法 ,而且为该细胞对病毒的敏感性提供了实验依据 ,并为该细胞的利用开辟了新的应用前景。

To culture human bone marrow stromal cells long term in vitro , and investigate their susceptibility to virus, the fetus, child and adult bone marrow stromal cell were cultured for long time in vitro by the adherent assay. The myoid bone marrow stromal cells were obtained after being generated more than five times, then their susceptibility to five kinds of virus was assayed by microdose CPE. All myoid stromal cells were susceptible to VSV, PolioV, HSV I and Ⅱ. Except CoxB 3, producing obvious CPE with TCID 50 up to 10 -3 10 -4 . Fetal myoid stromal cells were two times higher than child, and four times than adult in terms of virus susceptibility. Our research supplies not only a simple. practicable assay for ex vivo long term culture of human bone marrow stromal cell, but also experimental reference for estimating the virus susceptibility of these cells, at the same time, contributes to a more extensive prospect for applying these cells.