苜蓿丫纹夜蛾核多角体病毒复制与宿主细胞核骨架关系初探

Studies on Association of the Replication of AcMNPV with the Nuclear Matrix

用选择性抽提方法和整装细胞电镜技术观察苜蓿丫纹夜蛾多粒包埋核型多角体病毒 (AcM NPV)感染的Tn5B1细胞的核骨架结构体系 ,发现感染早期病毒的复制过程未对宿主细胞核骨架的形态结构产生明显影响 ,而感染晚期多角体的装配在核骨架网络中进行。以ie 1基因和多角体蛋白基因为探针 ,点杂交分析基因转录活性与宿主细胞核骨架的关系 ,结果表明在病毒感染早期 ,无论是正具转录活性的ie 1基因还是不具转录活性的多角体蛋白基因都可紧密结合在宿主细胞的核骨架上。

The structure of nuclear matrix in Tn5B1 cell infected with AcMNPV was observed by means of sequential cell fractionation and whole mount technique of electron microscopy. Whereas early infection of AcMNPV resulted in no dramatic change in the nuclear matrix morphologically, polyhedra formed in the network of the nuclear matrix in the late stage of infection. Results of Dot boltting showed that both ie 1 gene and polyhedrin gene tightly binded on the nuclear matrix in early stage of infection.