番木瓜环斑病毒PCR检测技术研究

Detection of Papaya Ringspot Virus by Immuno-capture PCR, Nested-PCR and ELISA-PCR Assays

建立了检测番木瓜环斑病毒 (PRV)的免疫捕捉 PCR (IC PCR)法、ELISA PCR法和巢式 PCR法 ,它们分别能从 10 - 4、10 - 7和 10 - 14 稀释度的番木瓜叶粗汁液 (所含鲜叶组织的量分别 0 .5ug、0 .5ng和 5× 10 - 6ng)中检测出PRV。

Sensitive, rapid, and reliable immuno capture PCR (IC PCR), ELISA PCR and Nested PCR assays were developed for the first time to detect PRV. It was detected from dilutions of 10 -4 , 10 -7 and 10 -14 (equivalent to 0.5 ug, 0.5 ng and 5×10 -8 ng of papaya leaf tissues) by IC PCR, ELISA PCR and Nested PCR, respectively.