盐逆境下转基因耐盐小麦与其受体呼吸途径的动态变化

Dynamics of respiration pathway in transgenic salt-tolerant wheat and its receptor under salt stress

以转基因耐盐品种 8912 2和其受体陇春 13号两种小麦为实验材料 ,研究了小麦幼苗在不同盐浓度胁迫下呼吸途径动态变化 .结果表明 ,8912 2出现盐呼吸明显迟于其受体 ;两品种的Valt与 ρValt变化并不同步 ,且Valt均受高盐浓度的抑制 ,但低盐浓度能诱导其受体的Valt;两品种的 ρValt与 ρ′Vcyt彼此协同调节适应盐逆境 ,且 ρ′Vcyt仍是盐胁迫过程中线粒体电子传递的主要途径 .同时讨论了盐逆境下抗氰呼吸的一些可能生理作用 ,提出抗氰呼吸运行的强弱作为筛选植物耐盐性的一个生理指标是可行的 .

Using transgenic salt tolerant wheat 89122 and its receptor Longchun 13 as test material, the dynamic changes of respiration pathway of wheat seedlings at different salt concentration were studied.The appearance of salt respiration was later in 89122 than in Longchun 13.The changes of V alt and ρV alt of both varieties had different patterns during salt stress process.V alt was suppressed by high salt levels, but could be induced by low salt levels in Longchun 13. The ρV alt and ρ′V cyt of two varieties were cooperatively regulated with each other to adapt to salt stress,and ρ′V cyt was the main electron transport pathway.The possible physiological roles of cyanide resistant respiration were also discussed.It was suggested that the operation intensity of cyanide resistant respiration could serve as a physiological index of plant salt tolerance.