苦丁茶愈伤组织的诱导与褐变抑制

CALLUS INDUCTION AND BROWNING INHIBITION IN ILEX KUDINGCHA C. J. TSENG

以叶片为材料，对苦丁茶愈伤组织的诱导，继代培养及褐变调控的研究表明：（１）苦丁茶愈伤组织的诱导及继代培养均以 ＭＳ附加 ＢＡ２．０ ｍｇ Ｌ－１和 ＮＡＡ ４．０ ｍｇ Ｌ－１的培养基效果最好；（２）０．１％的植酸可明显促进愈伤组织生长、抑制褐变，而硫代硫酸钠效果最差；（３）连续培养４０—５０ ｄ愈伤组织增长倍数达到最大值；（４）继代２７次以后愈伤组织生长速度开始下降．这些条件为下一步细胞培养生产苦丁茶甙等生物活性物质提供了基础．

Young leaf segments from micropropagated plants were used to study the optimum medium and growth regulators for callus induction of Ilex kudingcha. The highest multiplication rate for callus induction and subculture of calluses was obtained using MS medium supplemented with 2.0 mg L-1 BA and 4.0 mg L-1 NAA. 0.1% phytic acid could markedly promote the growth of calluses and inhibit callus browning. Multiplication rate of calluses was highest in calluses cultured for 40 - 50 days, and decreased after 27th generation in subculture.