摘要
实验采用微束激光穿刺技术和农杆菌介导方法 ,将携带有 型烟草β 1 ,3葡聚糖酶基因和 型菜豆几丁质酶基因的 p BLGC质粒导入马铃薯 ( Solanum tuberosum L .)品种“津引 8号”。微束激光穿刺技术转化中 ,获得了 7株卡那霉素抗性再生苗 ,分子检测证明 ,其中有 1株为菜豆几丁质酶基因单基因转化植株。在农杆菌介导方法中得到了 1 3株卡那霉素抗性再生苗 ,其中有 3株为烟草β 1 ,3葡聚糖酶基因单基因转化植株。实验证明 ,同常规农杆菌介导方法相比 ,微束激光穿刺技术更为简便有效 ,细胞损伤小 ,为马铃薯遗传转化提供了一个外源基因直接导入的新途径。
The binary vector pBLGC, harboring tobacco class Ⅰ β1,3glucanase and bean class Ⅰ chitinase, were successfully introduced into the microtuber discs of potato (Solanum tuberosum L.) cultivars 'Jinyin 8' by using the Laser microbeam Puncture Techniques(LMPT) and Agrobacteriummediated methods. In the LMPT transformation, 7 kanamycinresistant regenerated plantlets have been obtained and the PCR and Southern blot analyses confirmed that 1 plant was bean class Ⅰ chitinasepositive. In the parallel experiment of Agrobacteriummediated methods, 3 of 13 kanamycinresistant regenerated plantlets was tobacco class Ⅰ β1,3glucanasepositive according to PCR and PCRSouthern analyses. It proved that both methods were applicable for potato transformation and that the LMPT was more effective and has less celldamage.
出处
《激光生物学报》
CAS
CSCD
2000年第3期189-193,共5页
Acta Laser Biology Sinica
关键词
马铃薯
微束激光穿刺技术
抗真菌病基因
Solanum tuberosum,
Laser microbeam puncture techniques,
Chitinase β1,3glucanase