重性抑郁障碍患者他克莫司结合蛋白5基因多态性分析

Tacrolimus binding proteins 5 gene polymorphisms in major depressive disorder

目的 分析中国汉族重性抑郁障碍患者他克莫司结合蛋白5（FKBP5）基因多态性.方法 对符合《美国诊断与统计手册精神障碍（第4版）》（DSM-Ⅳ）重性抑郁障碍诊断标准的263例患者予以单一抗抑郁药治疗,疗程6周.根据汉密尔顿抑郁量表（HAMD）评分,在治疗6周末将患者分为有效组（HAMD减分率≥50％）和无效组（HAMD减分率＜50％）.入组时取患者血液标本提取DNA,采用高温连接酶检测反应法（LDR）检测FKBP5基因的rs3800373和rs1360780两个位点.结果 （1）有效组FKBP5基因rs3800373位点GG＋ GT、TT基因型频率分别为45.7％、54.3％,无效组分别为47.6％、52.4％,两组比较差异无统计学意义（x2=0.051,P＞0.05）；有效组FKBP5基因rs1360780位点CC、CT ＋TT基因型频率分别为55.6％、44.4％,无效组分别为51.2％、48.8％,两组比较差异无统计学意义（x2=0.256,P＞0.05）；（2）有效组和无效组的rs3800373-rs1360780两位点单倍型分布差异无统计学意义（P ＞0.05）；（3） rs3800373、rs1360780两位点不同基因型患者之间总不良反应发生率及恶心不良反应发生率比较差异均无统计学意义（P＞0.05）.结论 未发现中国汉族重性抑郁障碍患者不同疗效组患者FKBP5基因rs3800373、rs1360780两位点分布存在差异.

Objective Tacrolimus binding proteins 5 （FKBP5） gene polymorphisms were analyzed in Chinese Han major depressive disorder （MDD） patients.Methods 263 major depressive disorder （MDD） patients who met the Diagnostic and Statistical Manual of Mental Disorders 4th Edition （DSM-Ⅳ）criterion were given antidepressant drugs for six weeks.The patients were divided into two groups of the responder group and the non-responder group,according to the decreasing rate of score of HAMD ≥ 50%and 〈 50% respectively.Genomic DNA was extracted from the blood samples,when patients being enrolled.Two polymorphisms （rs3800373 and rs1360780） of FKBP5 gene were genotyped using the ligase detection reaction.Results （1）The distribution frequencies of the SNP of rs3800373 showed no significant differences between responder group （GG ＋ GT 45.7% ; TT 54.3%） and non-responder group （GG ＋ GT47.6% ;T T 52.4%） （x2 =0.051,P 〉 0.05）.The distribution frequencies of the SNP of rs1360780 showed no significant differences between responder group（CC 55.6% ; CT ＋ TF 44.4%） and non-responder group （CC 51.2% ; CT ＋ TT 48.8%） （x2 =0.256,P 〉 0.05）.（2） No significant differences were found in rs3800373-rs1360780 haplotype frequencies between responder group and non-responder group （P 〉 0.05）.（3） There were no significant differences in the incidence rate of total side effect and nausea adverse effect between the patients with different genotypes of rs3800373 and rs1360780 （P 〉 0.05）.Conclusions There may be no significant differences in the genotype frequencies of FKBP5 gene rs3800373 and rs1360780 polymorphisms between responder group and non-responder group in Chinese Han MDD patients.