用顺序GISH-FISH技术鉴定小麦-中间偃麦草小片段易位系

Characterization of a Wheat-Agropyron intermedium Translocation Line by Sequential GISH and FISH

利用顺序基因组－重复序列荧光原位杂交技术对１个来自中３不育系和普通小麦恢７５杂种后代稳定株系Ｈ９６２７６－２的染色体组成进行了分析。以中间偃麦草（Ａｇｒｏｐｙｒｏｎ ｉｎｔｅｒｍｅｄｉｕｍ）基因组ＤＮＡ为探针的荧光原位杂交结果表明，Ｈ９６２７６－２的体细胞中有４２条染色体，包括２０对小麦染色体和１对小麦－中间偃麦草易位染色体，中间偃麦草染色体的易位片段位于１对小麦染色体的端部。进而用重复序列探针 ｐＳｃ１１９进行第 ２次荧光原位杂交，证明 Ｈ９６２７６－２中的中间偃麦草染色体易位片段位于小麦２Ｂ染色体的短臂上。

The chromosome constitution on H96276-2, which is a stable line derived from a sterile wheat-Agropyron intermedium partial amphiploid Zhong 3 cross to common wheat Hui 75, was analyzed by sequential GISH and FISH. The results of GISH using genomic DNA of Agropyron intermedium as probe showed that H96276-2 possessed 20 pairs of wheat chromosomes and one pair of translocation chromosomes of wheat and Ag. intermedium. The translocated alien chromosome fragments located on the terminals of one pair of wheat chromosomes. Furthermore, reprobing of repeat sequence pSc119 was used to identify the wheat chromosomes involved in the translocation. It was determined that the alien fragments located in the terminals of the short arms of 2B chromosomes of wheat.