西瓜果实性状QTL定位及其遗传效应分析

Identification of Quantitative Trait Loci Associated with Fruit Traits in Watermelon [Citullus lanantus (Thanb) Mansf] and Analysis of Their Genetic Effects

用可溶性固形物含量高、薄皮、感枯萎病的栽培西瓜自交系（Ｃｉｔｒｕｌｌｕｓ ｌａｎａｔｕｓ ｖａｒ．ｌａｎａｔｕｓ） ９７１０３和可溶性固形物含量低、皮厚、抗病的野生西瓜种质（Ｃｉｔｒｕｌｌｕｓ ｌａｎａｔｕｓ ｖａｒ． ｃｉｔｒｏｉｄｅｓ） ＰＩ２９６３４１杂交所得Ｆ２的１１８个单株为作图群体，通过构建分子连锁图谱，对西瓜主要果实性状可 溶性固形物含量、果皮硬度、果皮厚度、单果重、种子千粒重进行区间作图，定位了影响可溶性固 形物含量的４个ＱＴＬ、影响果皮硬度的５个ＱＴＬ、影响果皮厚度的２个ＱＴＬ、影响单果重的３个 ＱＴＬ、影响种子千粒重的６个ＱＴＬ。此外，估算了每个ＱＴＬ的贡献率、加性效应与显性效应。为开 展西瓜果实性状改良分子标记辅助选择提供了理论基础。

In this study, we mapped and characterized quantitative trait loci (QTL) affecting watermelon fruit the traits . A total of 118 F2 progenies derived from the cross 97103 (which is a cultivar with higher total soluble solids concentration, thin rind, susceptible to Fusarium wilt disease) × PI296341(which is a wild germplasm with lower total soluble solids concentration, thick rind, resistant to Fusarium wilt disease) were used to construct a 96-markers map in watermelon. By using interval mapping 4 QTLs for total soluble solids concentration, 5 QTLs for hardness of rind, 2 QTLs for thickness of rind, 3 QTLs for weight of simple fruit, and 6 QTLs for weight of one thousand grain seeds were identified. In addition, the explained variations, additive effects and dominance effects for all detected QTLs were analyzed.