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PLLA/bpV(pic)微球的制备以及对神经细胞的作用

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摘要 目的制备出一种bpV(pic)的控制释放体系,并验证其对神经细胞的保护以及促进轴突生长的作用。方法用单乳化-溶剂挥发法制备出PLLA/bpV(pic)微球并对其进行表征。将神经干细胞以及背根神经节分别分为3组:普通培养基组、PLLA微球组、PLLA/bpV(pic)组,分别加入DMEM培养基,含有PLLA微球的DMEM培养基,以及含有PLLA/bpV(pic)微球的DMEM培养基。通过死活细胞鉴定以及轴突长度测量评价PLLA/bpV(pic)组微球对细胞的保护作用以及轴突生长的促进作用。结果 PLLA/bpV(pic)微球的成球率为(88.2±5.6)%,粒径大小为(16.8±3.1)%,载药量为(4.05±0.3)%,包封率为(48.5±1.8)%,释放时间可达30 d。PLLA/bpV(pic)组细胞存活率为(95.2±4.77)%,背根神经节轴突长度为(718±95)μm,均明显高于普通培养基组和PLLA微球组。结论初步试验结果证明采用单乳化-溶剂挥发法成功制备出PLLA/bpV(pic)微球,该微球对神经细胞存活以及背根神经节轴突生长有明显的促进作用。
作者 林强 李皓莘
机构地区 广东省中医院骨二科
出处 《广东医学》 CAS CSCD 北大核心 2013年第17期2626-2629,共4页 Guangdong Medical Journal
基金 广东省科技计划项目(编号:2011B031800205)
关键词 PLLA bpV（pic）微球 控制释放 神经干细胞 背根神经节
分类号 R651 [医药卫生—外科学]
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参考文献10

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广东医学
2013年 第17期

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