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二氯乙酸对NB4细胞株凋亡的诱导作用及机制

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摘要 目的 研究二氯乙酸对人急性髓系白血病NB4细胞的诱导凋亡作用并探讨其可能的作用机制.方法 用CCK-8法检测2~8 mmol/L二氯乙酸作用于NB4细胞48 h后增殖抑制作用;2~8 mmol/L二氯乙酸作用于NB4细胞24 h后,用流式细胞术分析细胞凋亡水平;Western blot检测凋亡相关蛋白细胞色素C、cleaved-caspase-3和cleaved-PARP的表达水平.结果 二氯乙酸对NB4细胞具有浓度和时间依赖性抑制增殖作用.二氯乙酸作用24 h后,线粒体释放到细胞浆的细胞色素C增加.结论 二氯乙酸能诱导NB4细胞凋亡,作用机制与释放线粒体细胞色素C激活caspase-3介导的细胞凋亡途径相关.
作者 刘伟才
机构地区 广东省佛山市三水区人民医院检验科
出处 《广东医学》 CAS CSCD 北大核心 2013年第18期2766-2768,共3页 Guangdong Medical Journal
关键词 二氯乙酸 NB4细胞 细胞色素C 肿瘤代谢
分类号 R733.710.5 [医药卫生—肿瘤]
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参考文献12

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广东医学
2013年 第18期

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