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糖尿病肾病尿毒症患者体内微炎症状态和外周血单个核细胞骨架蛋白的表达 被引量：4

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摘要 目的探讨2型糖尿病肾病(DN)尿毒症患者外周血单个核细胞(PBMC)骨架蛋白的表达及炎症细胞因子的水平。方法采用Ficoll淋巴细胞分离液密度梯度离心分离出PBMC,激光共聚焦技术检测研究对象(8例2型DN尿毒症患者和8例健康志愿者)PBMC骨架蛋的表达,并分别用ELISA法和免疫比浊法检测血清白细胞介素-6(IL-6)和C反应蛋白(CRP)浓度。结果 2型DN尿毒症患者外周血IL-6和CRP水平均高于健康志愿者(P<0.01),DN尿毒症患者PBMC可见细胞形态变形,肌动蛋白重排,细胞周边肌动蛋白丝带消失,出现明显的肌动蛋白收缩团块,有的细胞出现微丝溶解现象。结论 DN尿毒症患者体内存在微炎症状态,这一微炎症状态可能与PBMC骨架蛋白重排有关。
作者 杨孟雪 甘华 杨波 高琳 李显文
机构地区 遵义医学院附属医院内分泌科 重庆医科大学附属第一医院肾内科
出处 《广东医学》 CAS CSCD 北大核心 2013年第18期2814-2816,共3页 Guangdong Medical Journal
基金 重庆市卫生局2010年度医学科研计划项目(重点项目)(编号:2010-1-16)
关键词 2型糖尿病 尿毒症 单个核细胞 骨架蛋白
分类号 R512.62 [医药卫生—内科学]
  • 相关文献

参考文献9

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二级参考文献24

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二级引证文献9

广东医学
2013年 第18期

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