摘要
目的:建立冬青科植物铁冬青的HPLC指纹图谱。方法:采用反相高效色谱法,选用APOLLO C_8柱(250 mm×4.6 mm,5μm),乙腈-水为流动相梯度洗脱,采集时间55 min,流速1 ml·min^(-1)。结果:建立铁冬青药材的HPLC指纹图谱,标定铁冬青药材9个共有峰,方法学考察结果符合指纹图谱技术要求。结论:该方法稳定,准确,可靠,为铁冬青药材质量控制提供科学依据。
Objective: To establish the fingerprints of llex roundda Thunb. Method: A RP-HPLC method was used with an APOL- LO Cs (250 mmx4:61 mmx5 tLm)column and the mobile phase of acetonitrile-water. The analysis time was 55 minutes and the flow rate was l-0 ml .min-1 .Result: The HPLC fingerprints of llex roundda Thunb. were established, and 9 common characteristic peaks were markthe indices in,he methodological investigation met the relative requirement. Conclusion: The method is stable and reliable i'and can provide the scientific basis for the quality control of llex roundda Thunb.
出处
《中国药师》
CAS
2013年第9期1316-1319,共4页
China Pharmacist
基金
国家科技重大专项(编号:2012ZX09103201)
关键词
铁冬青
指纹图谱
HPLC
梯度洗脱
Ilexroundda Thunb.
Fingerprints
HPLC
Gradient elution