摘要
目的:建立HPLC法测定药用辅料柠檬黄的含量。方法:采用Agilent Zorbax SB-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.02 mol·L^(-1)乙酸铵溶液(20∶80)为流动相;流速为1.0 ml·min^(-1);检测波长为226 nm;柱温为35℃。结果:柠檬黄线性范围为0.050~1.000μg(r=0.999 9),平均回收率为99.9%(RSD=0.9%,n=6)。结论:该法简单、准确,可用于药用辅料柠檬黄的含量测定。
Objective： To establish an HPLC method for the determination of pharmaceutical excipient lemon yellow. Method ： An Agilent Zorbax SB-CIs column （250 mm x 4.6 mm ,5 μm）was used with the temperature of 35μ. The mobile phase consisted of meth- anol-0.2 mol . L-1 ammonium acetate solution （20： 80） and the flow rate was 1.0 ml . min-l. The detection wavelength was 226nm. Result： The calibration curve of lemon yellow was linear within the range of 0.050-1. 000 μg（ r = 0.999 9 ）, the average recovery was 99.9% and RSD was 0.9% （n =6）. Conclusion： The method is simple and accurate, and can be used to determine lemon yellow.
出处
《中国药师》
CAS
2013年第9期1336-1337,共2页
China Pharmacist
基金
湖南省科学技术厅科技计划重点项目(编号:2011SK2018)
