HPLC-ECD法检测血浆中儿茶酚胺含量

Detremination of Catecholamines in Plasma by HPLC-ECD

目的:建立一种准确可靠、易于临床推广的血液中去甲肾上腺素(NE)、肾上腺素(E)和多巴胺(DA)的检测方法。方法:采用活性溶剂萃取标本中儿茶酚胺,以3,4-二羟基苄胺(DHBA)为内标,利用离子对高相液相色谱及电化学检测器进行测定。结果:NE、E、DA的加样回收率分别为(95.3±6.7)%、(101.8±5.4)%和(100.3±5.7)%。批内和批间精密度分别小于11.23%和17.66%。该方法测定NE和E在0.5~16×10^(-9)g范围内,DA在1~16×10^(-9)g范围内含量与峰面积比呈良好的线性关系,相关系数为0.998 5~0.999 8,最低检测限分别为:NE 0.3×10^(-9)g,E 0.35×10^(-9)g,DA 0.7×10^(-9)g。结论:此法简单、准确、灵敏、特异性高,便于临床和实验室推广应用。

Objective： To develop a reliable, simple and sensitive method to determine catecholamine in plasma. Method： Cate cholamines in plasma was extracted by the active solvents, then measured by HPLC-ECD with DHBA as the internal standard. Result： The recovery＇of norcpinephrine （ NE）, epinephrine （E） and dopamine （ DA ） was （ 95.3 ± 6.7 ） %, （ 101.8 ± 5.4 ） % and （ 1 00.3 -＋ 5.7 ）% , respectively. The inter and intra-day RSD were below 11.23% and 17.66% , respectively. A good linear correlation was found between the content and the peak area within the range of 0.5-16 ng for NE and E, and 1-16 ng for DA, and the correlation coef- ficients were within the range of 0.998 5-0. 999 8. The lower limit of detection for NE, E and DA was 0.3ng, 0.35ng and 0.7ng, re- spectively. Conclusion： The established method is simple, accurate and specific, and can be used to detect catecholamines in the clinical samples.