紫外分光光度法测定5-氯甲基糠醛浓度

Determination of 5-Chloromethylfurfural by UV spectrophotometry

建立了紫外分光光度法测定5-氯甲基糠醛(5-Chloromethylfurfural,5-CMF)浓度的方法.结果表明,5-CMF在石油醚/乙酸乙酯溶液中的λmax为275 nm,εmax达105L/(mol/cm)以上,回归方程为y=0.116 5x-0.004 7,相关系数R为0.999;5-CMF的平均回收率为99.78%,相对标准偏差为0.45%.从其回收率、灵敏度、准确度和线型范围(1～10 mg/L)来看,紫外分光光度法均能够满足5-CMF的定量分析要求.

An efficient method for the determination of 5-Chloromethylfurfural （5-CMF） content by UV spectrophotometer was established. Experimental results showed that in mineral ether/ethyl acetate solu- tion, 5-CMF has the maximum absorption wavelength at 275 nm, and that the εmax was more than 10s L/ （moL/cm）. The regression equation was y = 0. 116 5x -0. 004 7, the correlation coefficients was 0. 999, the average recovery rate of 5-CMF was 99.78% and relative standard deviation （RSD） was O. 45%. The recovery rate, accuracy, precision and linear range （ 1 - 10 mg/L） met the requirements of analysis of trace components by UV spectrophotometer. This analytic procedure provides a simple, rapid and cheap method for 5-CMF content determination.