高效液相色谱法测定水稻植株、糙米和土壤中的多菌灵残留量

Determination of Carbendazim Residues in Plant, Brown Rice and Soil by HPLC

建立了多菌灵在水稻植株、糙米和土壤中残留量的高效液相色谱定量检测方法。水稻植株、糙米和土壤采用甲醇与0.1 mol/L HCl的混合液提取，石油醚、乙酸乙酯萃取净化，高效液相色谱（UV）检测。对样品中的多菌灵进行不同水平添加回收试验，方法的平均回收率为85.1%～99.7%，相对标准偏差（RSD）为0.5%～5.2%，最低检测浓度均为0.05 mg/kg。糙米中多菌灵的残留量低于我国制定的最大残留限量，分析方法的灵敏度、准确度和精密度均符合残留检测要求。

A new method was developed for residual analysis of carbendazim in plant, brown rice and soil by HPLC. The samples were extracted with methanol＋0.1 mol/L hydrochloric acid, cleaned up by petroleum ether and ethyl acetate, determined using HPLC equipped with UV detector. The average recoveries of the method ranged from 85.1%to 99.7%, the relative standard deviations were 0.5%-5.2%. The limits of quantification of carbendazim in rice samples were all 0.05 mg/kg. The residue of carbendazim in rice was below the MRL in China. The method of pesticide residue analysis achieved acceptable sensitivity, accuracy and precision.