肾茶水提取物的体外抑菌实验

Bacteriostatic experiment for aqueous extract of clerodendranthus spicatus in vitro

目的探讨肾茶水提取物对15种常见临床分离致病菌的体外抑菌效果。方法以大肠埃希菌、金黄色葡萄球菌、白色念珠菌等15种常见临床分离致病菌为受试菌,采用微量肉汤稀释法测定肾茶水提取物对它们的最低抑菌浓度(MIC)。结果肾茶水提取物对大肠埃希菌、肺炎克雷伯菌、不活跃大肠埃希菌、甲型副伤寒杆菌、赫尔曼埃希菌、铜绿假单胞菌、鲍曼不动杆菌及金黄色葡萄球菌均具有不同程度的抑菌作用,而对白色念珠菌、克柔念珠菌、葡萄牙念珠菌、光滑念珠菌、热带念珠菌及屎肠球菌、粪肠球菌无抑菌作用。结论肾茶水提取物对大肠埃希菌、肺炎克雷伯菌等革兰阴性杆菌及葡萄球菌有抑制生长作用,临床用于治疗此类细菌引起的泌尿系感染有一定前景。

Objective To discuss the bacteriostatic effects of clerodendranthus spicatus aqueous extract on 15 clinically isolated common pathogenic bacteria in vitro. Methods The experimental bacteria were 15 kinds of clinically isolated common bacteria such as escherichia coli, staphylococcus aureus, and blastomyces albicans. The minimum inhibitory concentration of clerodendranthus spicatus aqueous extract for above bacteria was detected by the broth micro dilution method. Results Clerodendranthus spicatus aqueous extract had different bacteriostatic effects on escherichia coli, klebsiella pneumonia, non-active escherichia coli ,paratyphosus A bacillus, escherichia hemanil, pseudomonas aeruginosa, baumanii, and staphylococcus aureus and had no bacteriostatic effect on blastomyces albicaus, candida krusei, Portugal monilia, candida glabrata, candida tropicalis, enterococcus faecium, and enterococcus faecalis. Conclusion The aqueous extract of clerodendranthus spicatus has inhibitive effect on the growth of gram negative bacilli and staphylococci such as escherichia coli and klebsiella pneumonia.