高效液相色谱法测定乳宁片中菝葜皂苷元含量

Determination of Sarsasapogenin Content in Runing Tablet by HPLC

目的建立测定乳宁片中菝葜皂苷元含量的高效液相色谱（HPLC）法。方法采用AgilentSBC18柱（250mm×4．6mm，5μm），以甲醇-水（91：9）为流动相，流速为0．8mL／min，应用蒸发光散射检测器检测，漂移管温度为80℃，气体流速为2．0mL／min。结果菝葜皂苷元进样量在0．7536～6．7824μg范围内与峰面积积分值对数呈对数线性关系（r：0．9984），平均回收率为99．22％，RSD=0．87％（n=6）。结论该法操作简便，专属性强，检测结果准确可靠，可用于乳宁片的质量控制。

Objective To estabalish a high performace liquid chromatography（HPLC） method for the content determination of sarsasa- pogenin in Runing Tablet. Methods The Agilent SB Ci8 column（250 mm×4.6 mm, 5 μm） was used with the mobile phase of methanol-water（91：9）. The flow rate was 0. 8 mL/min. The evaporative light scattering detector was adopted for the detection with the drift tube temperature of 80 ℃ and the gas flow rate of 2. 0 mL/min. Results The sample size of sarsasapogenin in the range of 0. 753 6- 6. 782 4 μg showed a good logarithmic linear relationship with the integral value of the peak area（ r =0. 998 4）. The average recovery rate was 99. 22%, RSD was 0. 87% （n =6）. Conclusion The method is simple to operate, strongly pecific, accurate and reli- able in the detection results, which can be used for the quality control of Runing Tablet.