红河州RhD阴性血液冷冻血库的建立及应用

Establishment and application of frozen blood bank for RhD negative blood in Honghezhou

目的:通过建立本地区RhD阴性献血者信息库,并用深低温保存技术制备RhD阴性红细胞冷冻储存,临床急救时解冻洗涤供应临床。方法:对我站无偿献血者采集的标本先进行RhD阴性初筛和复检,对确诊为RhD阴性者鉴定CcEe表型分型,创建RhD阴性献血者信息库;采集的阴性血制备成冰冻红细胞,应用于急救RhD阴性患者。结果:2005-2012年共筛查无偿献血者90 402例,共确认RhD阴性献血者292例(0.32%)。其中:A型90例(30.82%)、B型71例(24.32%)、O型101例(34.59%)、AB型30例(10.27%)。共制备RhD阴性红细胞287.75U,为临床急救提供RhD阴性红细胞173U。结论:在本辖区无偿献血者中筛查RhD阴性献血者并建立RhD阴红细胞冷冻血库,为本辖区RhD阴性患者的临床急救提供重要保障意义。

Objective： To build the information base for the RhD negative blood donor in this region and prepare RhD negative red blood cell refrigerated storage with cryopreservation technology for clinical trial by thawing and washing in time of clinical emergency. Method： The RhD negative samples which were detected and verified after the voluntary blood donors were collected,CcEe phenotype identification of the negative donors in the preliminary selection was divided,and the information base for the RhD negative blood donor was created. The acquired nega- tive blood was prepared into the freezing red blood cell and applied for treating the emergent RhD negative pa tients. Result：From 2005 to 2012,90 402 voluntary blood donors were selected, and it was confirmed that there were 292 RhD negative blood donors （0.32%）,in which there were 90 cases of A blood-type （30. 82%）, 71 cases of B blood type （24.32%） ,101 cases of O blood type （34.59%） and 30 cases of AB blood-type （10.27%）. About 287.75 U of RhD red blood cells were prepared,and 173 U were provided to the clinical emergency. Conclusion： There would be a great significance in selecting the RhD negative blood donors from the voluntary blood donors and establishing the frozen blood bank for RhD red blood ceils for the clinical emergency treatment of the RhD negative patients.