摘要
目的探讨本地区重症监护室多重耐药铜绿假单胞菌对β-内酰胺类抗生素的耐药特点及其OXA型耐药基因的分析。方法采用K-B法检测32株多重耐药铜绿假单胞菌对4种β-内酰胺类抗生素(氨曲南、头孢吡肟、亚胺培南和哌拉西林/三唑巴坦)的耐药情况;采用多重PCR法检测32株多重耐药铜绿假单胞菌的4种OXA型基因(OXA23、OXA24、OXA51和OXA58)。结果 32株多重耐药铜绿假单胞菌对β-内酰胺类抗生素耐药率分别为:氨曲南87.5%(28/32)、头孢吡肟62.5%(20/32)、亚胺培南59.38%(19/32)、哌拉西林/三唑巴坦56.25%(18/32)。32株多重耐药铜绿假单胞菌中有2株分别含bla OXA23基因和bla OXA51基因。结论重症监护室多重耐药铜绿假单胞菌对β-内酰胺类抗生素的耐药率较高,均在50%以上。bla OXA51基因在本地区首次检出,但其播散流行趋势可能不如bla OXA23基因。
Explore the features of 13-1actam antibiotic resistance and analyse OXA-type gene in multidrug-resistant Pseudomonas aeruginosa isolated from intensive care unit in this region. Methods K-B test was used to detect the rate of drug resistance in 32 multidrug-resistant Pseudomonas aeruginosa resisted to 4 13-1actam antibiotics(Aztreonam,Cefepime, Imipenem and Piperacillin/Tazobactam). 4 kinds of OXA-type genes(blaoxA23, blaoxa24, blaoxA51 and blaoxn58)were detected by multiplex PCR in 32 multi-drug resistant Pseudomonas aeruginosa. Results The rates of drug resistance in 32 multidrug-resistant Pseudomonas aeruginosa to 4 kinds of 13-1actam antibiotics were respectively aztreonam 87.5%(28/32),cefepime 62.5%(20/32), imipenem 59.38%(19/32), piperacillin/ tazobactam 56.25%(18/32); In 32 isolates of multidrug-resistant Pseudomonas aeruginosa, a strain contained blaoxA23 genes and another contained blaoxn51 gene. Conclusion β-1actam antibiotic resistance rate was higher than 50% in multi-drug resistant Pseudomonas aeruginosa isolated from intensive care unit. blaonoxa51 gene was detected for the first time, but its spreading tendency may not be so widely compared with blaoxa23 gene.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2013年第10期791-794,共4页
Chinese Journal of Antibiotics
基金
湖南省衡阳市科技局和财政局基金资助
[编号:衡财企(2012)399号J9]