生防菌盾壳霉抗药性突变菌株的选育

Breeding of fungicides tolerant mutants from bio-control fungus Coniothyrium minitans

通过对野生型盾壳霉茵株Coniothyrium minitans JN-CM的紫外诱变,分别获得了抗茵核净茵株UV-D1、UV-D2,抗福美双菌株UV-T和抗异茵脲菌株UV-I突变株。4株抗药茵株经连续10次传代后,具有良好的遗传稳定性。与野生型茵株相比,突变茵株对不同杀茵剂茵丝生长的EC_(50)分别提高了75.43(茵核净)、95.14(茵核净)、125.02(福美双)和13.74(异茵脲)倍,对孢子萌发的EC_(50)分别提高了165.01(茵核净)、475.14(茵核净)、245.67(福美双)和65.84(异茵脲)倍。突变菌株呈现良好的生长繁殖能力、酶学活性及致腐能力,具有良好的应用前景。

Four mutants （UV-D1, UV-D2, UV-T and UV-I） tolerant to three fungicides （Dimetachlone, Thiram and Iprodione） were obtained by ultraviolet mutagenesis from the wild-type strain C. minitans JN-CM. These mutants showed genetic stability after cultivation for 10 generations. Their ECs0 for the mycelia growth increased 75.43 times （Dimeta- chlone）, 95.14 times （Dimetachlone）, 125.02 times （Thiram） and 13.74 times （Iprodione） respectively, as well as for the conidial germination, 165.01 times （Dimetachlone）, 475.14 times （Dimetachlone）, 245.67 times （Thiram） and 65.84 times （Iprodione） respectively, if compared with those of the wild-type strain. The mutants showed good growth and reproductive performance, enzyme activity and higher parasitic activity to the sclerotia of Sclerotinia sclerotiorum.