癌痛消颗粒对移植性肝癌模型大鼠肝癌组织miRNAs表达的影响

The Effect on miRNAs of Replant Liver Cancer Rats Treated by Aitongxiao Granule

目的用大鼠Walker-256移植性肝癌模型研究癌痛消颗粒对大鼠肝癌miRNAs的调节作用,以探讨癌痛消颗粒抗肝癌的可能机制。方法将60只大鼠移植性肝癌模型随机分为5组,即癌痛消颗粒高剂量组(A组)、中剂量组(B组)、低剂量组(C组)、5-Fu组(D组)、生理盐水组(E组),于造模后第8天对癌痛消治疗组及生理盐水组灌胃给药,5-Fu组腹腔注射给药,连续用药14 d后停药24 h,处死大鼠,取出瘤块,用实时定量聚合酶链反应(qRT-PCR)技术观察肝癌特定miRNAs(miR-199a-3p、miR-199a-5p、miR-18)表达水平。另设正常饲养组(F组)大鼠10只,不进行造模及给药,供实验结束时作空白对照用。结果造模组与空白对照组比较miR-18有更高水平的表达,miR-199a-3p,miR-199a-5p表达水平降低;造模各组(A、B、C、D组)药物干预后与生理盐水组(E组)比较肝癌组织miR-18表达水平降低,miR-199a-3p,miR-199a-5p表达水平升高(P<0.01);和E组比较,A、B、C、D组均可明显调节miRNAs水平,癌痛消中剂量组比5-FU组更为明显(P<0.01)。结论口服癌痛消颗粒对大鼠移植性肝癌组织的miRNAs表达具有一定的调控作用,提示癌痛消的抗癌机制可能与调控肝细胞癌发生和发展相关的miRNAs水平有关。

OBJECTIVE To explore the effect of Aitongxiao granule （traditional Chinese medicines） therapy on miRNAs of re- plant liver cancer rats. METHODS The 60 replant liver cancer rats were randomly divided into high dose （ A）, middle dose （ B ）, lower dose（C）, 5-Fu（D） ,distilled water（E） groups. After 14 days of experimental treatment, the expressions of miR-199a-3p,miR- 199a-5p ,miR-18 in liver cancer tissues were detected with qRT-PCR. RESULTS Before experimental treatment,in comparison with E group, the level of miR-I 8 was higher but level of miR-199a-3p, miR-199a-5p were lower in A,B,C ,D groups （P 〈 0.01 ）. After 14 days of experimental treatment, in comparison with E group, the level of miR-18 was lower but level of miR-199a-3p, miR-199a-5p were higher in A ,B ,C ,D groups（ P 〈 0.01 ）. The more significant differences were found in B group than that in D group. CONCLU- SION The Aitongxiao granule has the regulative effects on miRNAs of replant liver cancer rats . Its therapeutical effects relate to the miRNAs expressions of liver cellular cancer development .