摘要
通过超滤与大孔树脂吸附分离黑豆蛋白酶解产物,获得降胆固醇活性较强的黑豆肽组分,采用凝胶过滤色谱(GFC)、反相高效液相色谱(RP-HPLC)与排阻色谱(SEC)对黑豆肽粗品进行纯化,得到高纯度的降胆固醇肽,经Edman降解、N端测序对降胆固醇黑豆肽一级结构进行分析鉴定,其氨基酸组成序列为Ala-Phe-Pro-Lys-Asp,相对分子质量为576,肽链中具有特定的疏水区域,且Pro位于N端之外的重要位点。
Black soybean peptides (BSPs) with strong hypocholesterolemic activity were purified from black soybean protein hydrolysates by using ultrafiltration (UF) and macroporous adsorption resin (MAR), and further purified using Sephadex G-25 column, reversed phase high-performance liquid chromatography (RP-HPLC) and size exclusion chromatography (SEC). The amino acid sequence of the peptide with the strongest hypocholesterolemic activity was confirmed as Ala-Phe-Pro-Lys-Asp by automated Edman degradation in a protein sequencer, and its relative molecular mass was determined to be 576 D. In addition, a specific hydrophobic domain in the sequence was observed, and Pro was located in an important region except the N-terminus.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第19期128-132,共5页
Food Science
关键词
黑豆肽
降胆固醇
分离
结构鉴定
black soybean peptides
hypocholesterolemic activity
isolation
structural analysis