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降胆固醇黑豆肽的分离纯化与结构鉴定 被引量:13

Isolation and Identification of Hypocholesterolemic Peptide Derived from Black Soybean Protein
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摘要 通过超滤与大孔树脂吸附分离黑豆蛋白酶解产物,获得降胆固醇活性较强的黑豆肽组分,采用凝胶过滤色谱(GFC)、反相高效液相色谱(RP-HPLC)与排阻色谱(SEC)对黑豆肽粗品进行纯化,得到高纯度的降胆固醇肽,经Edman降解、N端测序对降胆固醇黑豆肽一级结构进行分析鉴定,其氨基酸组成序列为Ala-Phe-Pro-Lys-Asp,相对分子质量为576,肽链中具有特定的疏水区域,且Pro位于N端之外的重要位点。 Black soybean peptides (BSPs) with strong hypocholesterolemic activity were purified from black soybean protein hydrolysates by using ultrafiltration (UF) and macroporous adsorption resin (MAR), and further purified using Sephadex G-25 column, reversed phase high-performance liquid chromatography (RP-HPLC) and size exclusion chromatography (SEC). The amino acid sequence of the peptide with the strongest hypocholesterolemic activity was confirmed as Ala-Phe-Pro-Lys-Asp by automated Edman degradation in a protein sequencer, and its relative molecular mass was determined to be 576 D. In addition, a specific hydrophobic domain in the sequence was observed, and Pro was located in an important region except the N-terminus.
出处 《食品科学》 EI CAS CSCD 北大核心 2013年第19期128-132,共5页 Food Science
关键词 黑豆肽 降胆固醇 分离 结构鉴定 black soybean peptides hypocholesterolemic activity isolation structural analysis
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参考文献23

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