摘要
以11株抗酸性和8株酸敏性植物乳杆菌为实验菌株,采用群体分组分析法(BSA)和随机扩增多态性DNA(RAPD)技术对55条引物进行筛选,并对基因池中扩增出差异性条带的引物进行单菌株验证,最终获得1个抗酸性RAPD标记:S116-680;将抗酸性RAPD特异片段回收,与pGEM-T Easy载体连接后测序;根据序列信息设计了一对特异性引物,该对引物能在11株抗酸性菌株中稳定扩增出一条680bp的特异性条带,命名为SA-680抗酸性相关标记。
Totally 11 acid-resistant Lactobacillus plantarum strains and 8 acid-sensitive Lactobacillus plantarum strains were investigated, and random amplified polymorphic DNA （RAPD） and bulked segregate analysis （BSA） techniques were employed in this study. One RAPD marker S116-680 related to acid resistance in Lactobacillus plantarum was obtained through the selection from 55 random primers and the confirmation in a single Lactobacillus plantarum strain. The specific fragments were recovered and ligated into pGEM-T Easy vector, and then sequenced. According to the sequence information, a pair of specific primers （SA1/SA2） were designed, and SA1/SA2 with stable amplification of a 680bp specific band from 11 acid-resistant strains, was named as SA-680 acid resistance-related marker.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第19期181-185,共5页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAD31B07)
中央高校基本科研业务费专项(2109021104)
国家现代农业产业技术体系建设专项(nycytx-30-ch-03)
