利用响应面法优化北冬虫夏草发酵米固态发酵条件

Optimization of Solid-state Fermentation of Rice by Cordyceps militaris by Response Surface Methodology

采用Plackett-Burman设计筛选出对北冬虫夏草发酵米有显著影响的3个因素:料水比、光照强度、光照时间;在此基础上进行最陡爬坡试验确定试验的最大响应区域,根据中心组合的原理设计响应面试验,得到北虫草发酵米最佳固态发酵条件:料水比5.19:1(m/V)、发菌温度25℃、接种量12mL/100g、光照强度980.13lx、光照时间9.19d,虫草素含量为64.1mg/100g。

Plackett-Burman design was applied to screen 3 significant ones （material/water ratio, light intensity and illumination time） from 5 potential key factors influencing the solid-state fermentation of rice by Cordyceps militaris. After the maximum response region was approached by steepest ascent path design, the significant factors were optimized by central composite design combined with response surface methodology. A material/water ratio of 5.19：1 （m/V）, temperature of 25 ℃, inoculum size of 12 mL/100 g, light intensity of 980.13 lx and an illumination time of 9.19 d were found to the optimum conditions to obtain a cordycepin yield of 64.1 mg/100 g.