慢性应激抑郁模型大鼠海马及胃肠黏膜水通道蛋白的表达

The expressions of aquaporins in chronic stress depression model rat hippocampus and gastrointestinal mucosa

目的观测水通道蛋白（Aquaporins，AQPs）在慢性应激抑郁模型大鼠组织中的表达，探讨抑郁症部分躯体症状的发病机制。方法采用慢性应激抑郁大鼠模型；将20只大鼠随机分为正常对照组和模型对照组，以免疫组化研究AQP4及AQP3、AQP8分别在抑郁模型大鼠海马及胃肠中的表达。结果正常组及模型组大鼠海马中AQP4平均光密度分别为0．28±0．02、0．22±0．06，2组比较差异具有统计学意义（t值为2．756，P〈0．05）；2组胃及结肠黏膜中AQP3的表达差异无统计学意义（t值为1．814，1．812，P〉0．05）。小肠黏膜中AQP3平均光密度值2组分别为0．15±0．02、0．17±0．02，差异有统计学意义（t值为2．769，P〈0．05）。2组AQP8表达的平均光密度值胃黏膜为0．15±0．01、0．19±0．04；小肠黏膜为0．16±0．01、0．21±0．04；结肠黏膜为0．16±0．01、0．22±0．04，差异有统计学意义（t值分别为3．139，5．113，4．534，P〈0．05，P〈0．01，P〈0．01）。结论抑郁模型大鼠海马中APQ4的表达低于正常组；胃及结肠黏膜上皮中AQP3表达无明显变化，小肠黏膜表达上调；AQP8在胃、小肠及结肠黏膜中的表达均上调。

Objective To study the mechanisms of depression by exploring expressional differences of AQPs in the tissues of chronic stress depression rats. Methods Depression model was replicated by unpredicted chronic stress. 20 rats were randomly separated into normal control group and model control group, AQP4 and AQP3, AQP8 expressions on hippocampus and gastrointestinal mucosa of rat model with depression were detected by immunochemieal staining method. Results Means of optical density of AQP4 of normal group and model group hippocampus were 0.28 ± 0.02,0.22 ± 0.06 respectively, and the difference between two groups was significant statistically（ t value was 2. 756, P〈 0.05 ）. The expression of AQP3 on gastric mucosa and colonic mucosa between two groups had no significant statistically（ t value were 1. 814,1. 812, P 〉 0.05 ）. Two groups＇means of optical density of AQP3 on small intestinal mucosa were 0.15 ± 0.02,0.17 ± 0.02, and the difference was significant sta- tistically（ t value was 2. 769, P〈 0.05 ）. Two groups＇means of AQP8 optical density in gastric mucosa were 0.15 ± 0.01,0.19 ± 0.04 ;0.16 ± 0.01 and 0.21 ± 0.04 in small intestinal mucosa ;0, 16 ± 0.01 and 0.22 ± 0.04 in colonic mucosa, and the differences were significant statistically （ t values were 3.139,5.113,4. 534, P 〈 0.05, P 〈 0.0l, P 〈 0.01 ）. Conclusion The expression of AQP4 on depression model rat hippocampus are lower than those of the normal group ; mad the expression of AQP3 on gastric mucosa and colonic mucosa are no change obvi- ously,but itg up-regulation on small intestinal mucosa. The expressions of AQP8 on gastric mucosa and small in- testinal mucosa and colonic mucosa are up-regulating.