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不同饲养环境对局灶性脑梗死大鼠学习记忆及胆碱乙酰转移酶的影响 被引量:1

Effects of different environmental intervention on learning-memery ability and choline acetyltransferase expression in rats after unilateral local cerebral infarction
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摘要 目的观察不同环境设置对局灶性脑梗死大鼠学习记忆能力及梗死灶周围胆碱乙酰转移酶(ChAT)表达的影响。方法将SD大鼠用电凝法造成右侧大脑中动脉阻塞模型,再分为对照组(每组5只,群居于标准笼)、探索学习组(居于迷宫笼)和丰富环境组(居于丰富环境笼)。24d时利用水迷宫试验测试大鼠学习记忆能力,28d时处死,免疫组化染色测定ChAT在梗死灶周围皮质的表达情况。结果丰富环境组大鼠逃避潜伏期最短(26.9±4.8)S,跨过原平台位置次数最多(6.1±1.1)次,其次是探索学习组[(33.6±5.1)S,(4.8±1.2)次],对照组时间最长[(41.4±6.4)s,(3.3±0.9)次],各组之间比较有显著差异。丰富环境组大鼠梗死灶周围ChAT表达(40.3±8.4)明显高于探索学习组(35.6±7.8)和对照组(25.4±6.5)。结论丰富环境设置可改善局灶性脑梗死大鼠的学习记忆能力,上调梗死灶周围皮层CHAT的表达。 Objective To observe the effects of different environmental intervention on the learning-mem- ory ability and choline acetyltransferase (CHAT) expression around the infarct in rats with unilateral local cerebral infarction. Methods SD rats were subjected to electric coagulation of MCA and then were assigned randomly to (1)control group(5 in each group housed in a standard cage) ,(2)learn training group( housed in a maze cage), (3) enriched environment group (housed in a enriched environment cage). 24 days later, rats were trained by water maze, and then killed for immunohistochemistry staining. The expression of ChAT in peri-ischemic cortex was ex- amined. Results The escape latency of rats in enriched environment was the shortest ( ( 26.9 ± 4.8 ) s), and the times striding over the platform was the most(6.1 ± 1.1 ) ,longer (33.6 ± 5.1 )s and less (4.8 ± 1.2)in the learn training group, the longest ( ( 41.4 ± 6.4 ) s) and least ( 3.3 ± 0.9 ) in the control group. The positive cells of ChAT in enriched environment group (40.3 ± 8.4 ) were higher than those in learn training group ( 35.6 ± 7.8 ) and those in control group(25.4 ± 6.5 ). Conclusion Enriched environment after MCAO could promote the learning-memo- ry ability and up-regulate the expression of ChAT in the peri-ischemic regions.
出处 《中华行为医学与脑科学杂志》 CAS CSCD 北大核心 2013年第9期797-798,共2页 Chinese Journal of Behavioral Medicine and Brain Science
关键词 丰富环境 学习记忆 胆碱乙酰转移酶 大脑中动脉阻塞 Enriched environment Learning-memory Choline acetyhransferase MCAO
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参考文献9

  • 1Ruscher K, Johannesson E, Brugiere E, et, al. Em'iehed envirnment reduces apolipoprotein E(ApoE) in reactive astrocytes and attenuates inflammation of the peri-infaret tissue after experimental stroke. J Ccreb Blood Flow Metab,2009,29 : 1796-1805.
  • 2Risedal A, Mattsson B, Dahlqvist P,et al. Environmental influences on funetional outcome afler a cortieal infanct in the rat. Brain Res Bull, 2002,58:315-321.
  • 3Chopra K, Misra S, Kuhad A. Neurobiological aspects of Alzheimer. Expert Opin Ther Targets,2011,15:535-555.
  • 4Bederson JB, Pitis LH ,Tsun M,et al. Rat Middle cerebral artery occlu- sion :evaluation of the model and development of a neurologic examin- ation. Stroke, 1986,17:472-476.
  • 5齐亚超,肖向建,段瑞生,张晓玲,郭宗成.不同饲养环境对局灶性脑梗死大鼠行为学恢复的影响[J].脑与神经疾病杂志,2012,20(6):460-462. 被引量:1
  • 6李春禄,张佳婧,王冬梅,李勇辉.丰富环境对野生小家鼠和昆明小鼠空间学习记忆能力的影响[J].中华行为医学与脑科学杂志,2011,20(7):624-626. 被引量:9
  • 7Biernaskie J, Corbett D. Enriched rehabilitative training promotes im- proved forelimb motor function and enham'ed dendritic growlh after fo- cal ischemic injury. J Neurosci,2001,21:5272-5280.
  • 8Sharp SI, Francis PT, Elliott MS, et al. Choline acetyltransferase activi- ty in vascular dementia and stroke. Dement Geriatr Cogn Disord, 2009,28:233 -238.
  • 9尹军祥,田金洲,程龙,时晶,刘峘,宋崇顺,任映,黄启福,王永炎.MCAO模型大鼠学习记忆特征[J].中国行为医学科学,2006,15(3):197-199. 被引量:8

二级参考文献20

  • 1程明,朱熊兆.学习记忆的行为学研究方法[J].中国行为医学科学,2005,14(1):65-66. 被引量:30
  • 2柯荔宁,王玮,张更,赵小贞.不同脑缺血方式对大鼠学习记忆能力的影响[J].中国行为医学科学,2005,14(10):879-881. 被引量:12
  • 3Tatemichi TK,Desmond DW,Mayeux R,et al.Dementia after stroke:baseline frequency,risks,and clinical features in a hospitalized cohort.Neurology,1992,42:1185-93.
  • 4Hagek VE.Brief assessment of cognitive impairment in patients with stroke.Arch phys Med.Rehabic,1989,70:114.
  • 5Tamura A,Graham DI,McCulloch J,et al.Focal cerebral ischemia in the rat:1.Description of technique and early neuropathological consequences following middle cerebral artery occlusion.J Cereb Blood Flow Metab,1981,1:53-60.
  • 6Z Longa EZ,Weinstein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke,1989,20:84-91.
  • 7Kollmar R,Schabitz WR,Heiland S,et al.Neuroprotective effect of delayed moderate hypothermia after focal cerebral ischemia:an MRI study.Stroke,2002,33:1899-1904.
  • 8Tamura M,Aoki Y,Seto T,et al.Cerebroprotective action of a Na+/Ca2+ channel blocker NS-7.II.Effect on the cerebral infarction,behavioral and cognitive impairments at the chronic stage of permanent middle cerebral artery occlusion in rats.Brain Res,2001,890:170-176.
  • 9Aoki Y,Tamura M,Itoh Y,et al.Cerebroprotective action of a Na+/Ca2+ channel blocker NS-7.I.Effect on the cerebral infarction and edema at the acute stage of permanent middle cerebral artery occlusion in rats.Brain Res,2001,890:162-169.
  • 10Morris R.Developments of a water-maze procedure for studying spatial learning in the rat.J Neurosci.Methods,1984,11:47.

共引文献15

同被引文献19

  • 1吴冰洁,顾平,崔冬生,耿媛,王铭维.丰富环境对快速老化小鼠SAMP8学习记忆能力的影响[J].第二军医大学学报,2007,28(9):964-967. 被引量:9
  • 2de Magalhaes JP, Wuttke D, Wood SH, et al.Genome-environment in- teractions that modulate aging: powerful targets for drug discovery [ J ]. Pharmacol Rev, 2012,64( 1 ) : 88-101.
  • 3Hu YS, Xu P, Pigino G, et al. Complex environment experience res- cues impaired neurogenesis, enhances synaptic plasticity, and attenu- ates neuropathology in familial Alzheimer' s disease-linked APPswe/ PS1Deha E9 mice[ J] .FASEB J,2010,24(6) : 1667-1681.
  • 4Yuan ZY, Gu P, Liu L, et al. Neuroprotective effects of enriched envi- ronment in MPTP-treated SAMP8 mice [ J ]. Neurosci Lett, 2009,454 ( 1 ) :6-10.
  • 5Kang L,Li S,Xing ZG,et al. Dihydrotestosterone treatment delays theconversion from mild cognitive impairment to Alzheimer's disease in SAMP8 mice[J].Horm Behav,2014,65 (5) :505-515.
  • 6Zilkova M,Koson P,Zilka N. The hunt for dying neurons:insight into the neuronal loss in Alzheimer' s disease [ J ]. Bratisl Lek Listy, 2006, 107(9-10) :366-373.
  • 7van Praag H, Kempermann G, Gage FH. Neural consequences of envi- ronmental enrichment[ J] .Nat Rev Neurosci ,2000,1 (3) : 191-198.
  • 8Brown J, Cooper-Kuhn CM, Kempermann G, et al. Enriched environ- ment andphysical activity stimulate hippocampal but not olfactory bulb neurogenesis[J] .The Eur J Neurosci,2003,17(10) : 2042-2046.
  • 9Kobilo T, Liu QR, Gandhi K, et al. Running is the neurogenic and neurotrophic stimulus in environmental enrichment [J]. Learn Mem, 2011,18(9) :605-609.
  • 10Herring A, Ambree O, Tomm M, et al. Environmental enrichment en- hances cellular plasticity in transgenic mice with Alzheimer-like pa- thology [ J ]. Exp Neurol, 2009,216 ( 1 ) : 184-192.

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