响应面分析南方红豆杉枝条多糖超声提取工艺及其抗氧化作用研究

Study on ultrasonic extraction of polysaccharides from branches of Taxus mairei (Lemee et Levl.) S. Y. Hu ex Liu by response surface method and its antioxidant activity

目的:研究南方红豆杉枝条中多糖的超声提取工艺及抗氧化作用。方法:在单因素实验基础上,采用响应面分析法对影响南方红豆杉枝条中多糖提取率的主要因素进行优化,建立影响因素与响应值之间的数学模型,确定最佳超声提取工艺条件;通过DPPH自由基清除率、还原力和总的抗氧化活性的测定,分析其抗氧化作用。结果:最佳超声提取工艺为:料液比26.78(V/W),提取时间42.81min,超声频率为33.88kHz,提取2次;南方红豆杉多糖对DPPH自由基具有一定的清除作用,总的抗氧化活性和还原能力均随多糖浓度的增加而上升。结论:采用该方法对提取条件进行优化,合理可靠,能为生产工艺提供理论依据;其多糖具有较强的抗氧化作用。

Objective： The research aimed to optimize the extraction technology of polysaccharides from the branches of Taxus mairei （Lemee et Levi.） S.Y. Hu ex Liu and study its antioxidant activity in vitro. Methods： Based on the single factor experiment, the main elements （the ratio between liquid and solid, the extraction time, and the ultrasonic frequency） affecting the extraction rate of polysaccharides from the branches of Taxus mairei （Lemee et Levl.） S. Y. Hu ex Liu were optimized by using response surface analysis, and the functional relationship between the response value and elements was established. The antioxidant activity of the polysaccharides in vitro was evaluated by the methods of scavenging DPPH radical, reducing power and total antioxidant capacity. Results： The result of the experiment showed the optimum extraction condition as follow： 26.78 times of water, extracting 42.81min by 33.88kHz ultrasonic frequency two times. The scavenging capacity of DPPH, reducing power and total antioxidant activity increased with the increasing concentration of polysaccharides. Conclusion： It is feasible and reliable tO optimize the conditions of extracting the polysaccharides from the branches of Taxus mairei （Lemee et Levi.） S. Y. Hu ex Liu by response surface analysis, which lays the theoretical foundation to its production process. The polysaccharides have strong antioxidant capacity in vitro.