摘要
目的:研究含耳叶牛皮消苷A(AA)血清对肿瘤细胞的抑制作用及细胞形态变化。方法:选择SD大鼠,采用灌胃方式给予AA,待血药浓度到高峰时取血,MTT法确定含药血清对多种肿瘤细胞的生长抑制作用,并通过倒置显微镜和荧光显微镜观察细胞的形态学的改变。结果:含AA血清对多种肿瘤细胞均具有抑制作用,并在倒置显微镜和荧光显微镜下可以看到显著的细胞凋亡形态学变化。结论:经代谢后的AA血清仍具有抑制肿瘤细胞生长作用,且具有诱导肿瘤细胞凋亡的能力。
Objective : To investigate the growth inhibitory effect of tumor ceils by auriculoside A (AA) serum. To observe the morphological changes of the cancer cells under inverted microscope and fluorescent microscope. Methods : The sera of SD rats were collected after intragastric administration of AA. The effect of the drug - serum on the proliferation of several tumor cell lines was analyzed with MTY assay. Morphological changes of the cancer cells were observed under inverted microscope and fluorescent microscope. Results: The drug serum had significant inhibition effect on several cancer cell lines. Obvious morphological changes of apoptosis can be observed under inverted microscope and fluorescent microscope. Conclusion : The serum after AA bio - transforrnated in body still has the anti - tumor effect and the ability of apoptosis - inducing.
出处
《中华中医药学刊》
CAS
2013年第10期2247-2248,I0001,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省医药卫生科学研究基金资助项目(2011KYA007)