海南黎汉族人群中高血压与ACE基因多态性的研究

Association between polymorphism of angiotensin-converting enzyme gene and hypertension in Li and Han Nationality in Hainan

目的探讨海南黎族和汉族人群血管紧张素转换酶(ACE)基因多态性与高血压的关系。方法于2010年对三个市县筛选高血压者85例作为病例组,血压正常者119例作为对照组,黎族101例,汉族103例。应用多聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测ACE基因的插入/缺失(I/D)多态性。用χ2检验,比较不同组人群ACE基因I/D多态性基因型频率和I、D等位基因频率的差异。结果在黎族EH组ACE各基因型的分布频率为II型44.7%、ID型34.2%、DD型21.1%,D等位基因频数38.2%;黎族NT组为II54.0%、ID33.3%、DD12.7%,D等位基因频数29.4%;黎族两组I/D基因型和D等位基因频数分布差异均无统计学意义(P>0.05)。汉族EH组三种基因型比例分别是II53.2%、ID10.6%、DD36.2%,D等位基因频数28.7%,汉族NT组II57.1%、ID28.6%、DD14.3%,D等位基因频数28.6%。汉族两组I/D基因型和D等位基因频数分布差异均无统计学意义(P>0.05)。结论海南黎族和汉族地区人群ACE基因I/D多态性与原发性高血压的发生无关联。

Objective To explore the association between polymorphism of angiotensin - converting enzyme （ACE） gene and hypertension in Li and Han Nationality in Hainan. Methods The.polymerase chain reaction-restriction fragment length polymorph （PCR-RFLP） technique was, based on the prevalence survey in 3 countris in 2010, used to examine the insertion/ deletion polymorphism of ACE gene. in Li and Han Nationality in Hainan.Chi square test was used to compare the differences of U D polymorphism genetypes frenqeney and I/D allele frequency among the two groups. Results The frequencies of II, ID , DD genotypes and D alleles were 44.7% ,34.2% ,21.1% and 38.2%, respectively in EH of Li nationality; NT of Li nationality＇ s were 54.0%, 33.3%, 12.7%, 29.4%, respectively; EH of Han nationality＇ s were 53.2%,10.6%,36.2% and 28.7%, respectively; NT of Hart nationality＇ s were 57.1%,28.6%,14.3% and 28.6%, respectively. There was no significant difference in frequencies of both genotypes and alleles between hypertensive group and normotensive group even Li nationality and Han nationality.. Gonclusion The I/D polymorphism of ACE gene was not related to hypertension of Li nationality and Han nationality in Hainan p.