摘要
Objective: Gallbladder carcinoma was one of the malignant tumors in the digestive system, characterized by high recurrence and invasion. Recent research indicates that chemotactic factors such as IL-8, MCP-1 and MIP-1α have played an important role in such aspects as formulation, growth, shifting of the tumor. The aim of this study was to investigate expressions of IL-8, MCP-1 and MIP-1α in gallbladder adenocarcinoma tissues. Methods: Gallbladder adenocarcinoma and noncancerous tissues were routinely formalin-fixed and paraffin-embedded, and in situ hybridization assay for IL-8, MCP-1and MIP-1α mRNA. Results:(1) The positive rates or the scorings of IL-8, MCP-1 and MIP-1α mRNA were significantly higher in human gallbladder adenocarcinoma than those in human chronic cholecystitis(P < 0.01). The positive rates or the scorings of three factors were lower in well-differentiatiated gallbladder adenocarcinoma than in poorly-differenfiatted ones, whereas there was only one significant difference between MCP-1 mRNA(P < 0.05). The closely positive correlation were found among IL-8, MCP-1 and MIP-1α mRNA.(2) Both the positive rates of IL-8 mRNA and MCP-1 mRNA as well as their scorings were tightly related to their invasion of the common bile duct and the occurrence of lymph node transfer, moreover, the positive rates of MIP-1α mRNA and its scorings were tightly related to its invasion of liver.(3) Close positive correlation exists not only in IL-8 mRNA and MCP-1 mRNA(r = 0.528), but also in MIP-1α mRNA and IL-8 mRNA(r = 0.422), so does in MCP-1mRNA and MIP-1α mRNA(r = 0.638). Conclusion: The positive rates or the scorings of IL-8, MCP-1 and MIP-1α mRNA are significantly higher in human gallbladder adenocarcinoma than those in human chronic cholecystitis, and the closely positive correlation are found among them, which suggests that IL-8, MCP-1 and MIP-1α regulate and influence the development and transformation of the gallbladder adencarcinoma together.
Objective：Gal bladder carcinoma was one of the malignant tumors in the digestive system, characterized by high recurrence and invasion. Recent research indicates that chemotactic factors such as IL-8, MCP-1 and MIP-1αhave played an important role in such aspects as formulation, growth, shifting of the tumor. The aim of this study was to investigate expressions of IL-8, MCP-1 and MIP-1αin gal bladder adenocarcinoma tissues. Methods：Gal bladder adenocarcinoma and noncancerous tissues were routinely formalin-fixed and paraf in-embedded, and in situ hybridization assay for IL-8, MCP-1 and MIP-1αmRNA. Results：（1） The positive rates or the scorings of IL-8, MCP-1 and MIP-1αmRNA were significantly higher in human gal bladder adenocarcinoma than those in human chronic cholecystitis （P〈0.01）. The positive rates or the scorings of three factors were lower in wel-dif erentiatiated gal bladder adenocarcinoma than in poorly-dif erenfiatted ones, whereas there was only one significant dif erence between MCP-1 mRNA （P〈0.05）. The closely positive correlation were found among IL-8, MCP-1 and MIP-1αmRNA. （2） Both the positive rates of IL-8 mRNA and MCP-1 mRNA as wel as their scorings were tightly related to their invasion of the common bile duct and the occurrence of lymph node transfer, moreover, the positive rates of MIP-1αmRNA and its scorings were tightly related to its invasion of liver. （3） Close positive correlation exists not only in IL-8 mRNA and MCP-1 mRNA （r=0.528）, but also in MIP-1αmRNA and IL-8 mRNA （r=0.422）, so does in MCP-1 mRNA and MIP-1αmRNA （r=0.638）. Conclusion：The positive rates or the scorings of IL-8, MCP-1 and MIP-1αmRNA are significantly higher in human gal bladder adenocarcinoma than those in human chronic cholecystitis, and the closely positive correlation are found among them, which suggests that IL-8, MCP-1 and MIP-1αregulate and influence the development and transformation of the gal bladder adencarcinoma together.
