共代谢基质对芽孢杆菌Y-4降解异喹啉的影响

Influence of co-metabolism substrates on isquinoline biodegradation by bacillus Y-4

以吡啶，葡萄糖和邻苯二甲酸作为共代谢基质，研究了它们对芽孢杆菌Y_4降解异喹啉的影响。实验结果表明各降解过程均遵循二级反应动力学方程：-dS／dt=K2S2＋K1S＋K0。吡啶的加入会抑制异喹啉的降解，并且吡啶的浓度越高，抑制作用越明显。反应体系中葡萄糖的浓度为100-800mg／L时，葡萄糖的加入会促进异喹啉的降解，且葡萄糖浓度越大，异喹啉降解速率P越大，当葡萄糖的浓度为800mg／L时，其降解率速率P可由未加葡萄糖的0．1924h。上升为0．2255h-1。适宜浓度的邻苯二甲酸会对异喹啉的降解产生促进作用，邻苯二甲酸的浓度为50mg／L时，异喹啉的降解速率可由原来的0．1924h-1增加到0．2145h-1，邻苯二甲酸浓度过高反而会抑制异喹啉的降解。

To promote the biodegradation ability of isquinoline, the effect of co-metabolism substrates on degradation of isquinoline by bacillus Y-4 was investigated. The substrates include pyridine, glucose and phthal- ic acid. The results demonstrated that the second-order equation -dS/dt = K2S2 ＋ KIS ＋ Ko was applied to the ex- perimental data obtained from the biodegradation test. pyridine, and the inhibition became apparent with the dation ability of isquinoline was gradually promoted by Isquinoline degradation was inhibited by the presence of increasing of the concentration of pyridine. The biodegra- the presence of glucose （ 100 - 800 mg/L）. The rate con- stant of isquinoline （450 mg/L） biodegradation was 0. 1924 h-1 in the absence of glucose, which increased to 0. 2255 h-1, respectively, when the glucose concentrations was 800 mg/L. In presence of phthalic acid and isquinoline, phthalic acid with the proper concentration enhanced isquinoline biodegradation ability. When the initial concentration of phthalic acid was 50 mg/L, the rate constant of isquinoline increased to 0. 2145 h-1 The high concentration isquinoline inhibited isquinoline degradation.