摘要
本文以丙烯酰胺作为功能单体、乙二醇二甲基丙烯酸酯作为交联剂、偶氮二异丁腈作为引发剂作为反应体系,分别向该体系中加入一定量的醋酸镍、醋酸铜和氯化锌以获得对蛋白质具有亲和力的活性位点,采用方便易行的热引发本体聚合方法制备了对蛋白质具有特异性吸附的三种掺金属离子聚合物,并通过透射电子显微镜和马尔文激光粒度仪对其进行了表征。随后用牛血清白蛋白(BSA)为蛋白质模型,考察了这三种掺金属离子聚合物对蛋白质的吸附性能。结果表明,这三种掺金属离子聚合物对蛋白质的吸附是由聚合物中的金属离子含量决定的,属于特异性的亲和吸附,且掺铜离子聚合物的吸附性能最强。
In this study, three kinds of metal ion-embedded polymers were synthesized by using a simple bulk polymerization way. Acrylamide (AM) served as functional monomer, ethylene glycoldimethacrylate (EGDMA) as cross-linker and 2,2-azoisobuty-ronitrile(AIBN) as initiator during this procedure. Then adequate nickelous acetate, copper acetate and zinc chloride was respective ly added to the reaction system to achieve active sites which could specifically adsorb protein. The polymer was characterized by transmission electron microscope and zetasizer nano. By using bovine serum albumin (BSA)as a model protein, we have investigated adsorption behavior between the metal ion-embedded polymer and protein. To identify the optimal adsorption solution, preliminary data showed that these three kinds of metal ion-embedded polymers do have particular adsorption for protein in pure water, and the Cu(II)-embedded polymer showed the best adsorption ability.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2013年第10期1435-1439,共5页
Chemical Research and Application
基金
国家自然科学基金(31100981)
高等学校博士学科点专项科研基金(20110182120014)
关键词
掺金属离子聚合物
牛血清白蛋白
金属离子亲和色谱
metal ion-embedded polymer
bovine serum albumin(BSA)
immoblized metal ion affinity chromatogaraphy