亚麻粗纱碱性脱胶菌株的筛选鉴定及脱胶效果

Screening and identification of alkalophilic degumming strains of flax roving and evaluation of its effects

以东海舟山海域腐烂海草为原料，筛选出碱性脱胶菌株DA8．对菌株DA8进行形态、生理生化和16SrDNA序列分析，分类鉴定其为纤维单胞菌（Cellulomonas sp）．酶活测定表明，该菌液同时具有果胶酶活力和木聚糖酶活力，并在pH为6．5-9．0、50℃以下酶活较稳定．利用该菌株在碱性条件下对亚麻粗纱进行脱胶，结果表明，菌株DA8对亚麻具有较好的脱胶效果，强力损伤小（下降14．19％）；扫描电镜表明，和未处理亚麻纤维以及传统化学处理纤维相比，DA8处理后亚麻纤维表面胶质去除干净且表面光滑．

Alkalophilic degumming strains DA8 was screened from the rotten wrack around Zhoushan ar- chipelago sea area. It was characterized as Cellulomonas sp by testing the strain morphology, physiology, bio- chemistry and the 16S rDNA sequences. Determination of enzyme activity showed that the activities of petin- ase and xylanase were obtained, and enzyme activities were stable under 50 ℃ and at pH 6.5-9.0. It was uti- lized to degum the flax roving under alkaline condition, and the result showed that the strain had a good result of degumming, and less strength with 14.19% of loss. Scanning electron microscopy （SEM） showed that com- pared with the untreated fiber and degummed by NaOH, the gum on the surface of flax fiber was mostly re- moved, and the surface of the flax fiber was smooth.