ADS系列大孔吸附树脂纯化夏枯草穗总黄酮工艺研究

Purification of Flavonoids from Prunella with ADS Type of Macroporous Resin

目的研究ADS系列大孔吸附树脂分离纯化夏枯草穗总黄酮的工艺条件及参数。方法以树脂对夏枯草穗总黄酮的吸附量和洗脱率为指标,筛选ADS系列大孔吸附树脂分离纯化夏枯草穗总黄酮的工艺条件。结果 ADS-8型大孔吸附树脂对夏枯草穗总黄酮有较好的吸附分离性能,该树脂分离纯化夏枯草穗总黄酮的最佳工艺条件为:上柱液pH值5.0,1.20mg/mL夏枯草提取液以流速2.0 BV/h上柱,洗脱剂乙醇体积分数40%,以1.5 BV/h的流速洗脱,收集洗脱液。经上述工艺纯化后,产品总黄酮含量达82.7%,收率66.2%。结论 ADS-8型大孔吸附树脂适于分离纯化夏枯草穗总黄酮。

Objective To investigate the technical parameters of purification process of total flavonoids from Prunella with ADS type of macroporous resin. Methods The conditions of purification of total flavonoids from Prunella were screened with adsorption capacity and desorption rate of total flavonoids as indicators. Results ADS-8 was the best resin for separation and purification offlavonoids from Prunella. The optimum conditions were： 1.20 mg/mL extract of Prunella at the pH value of 5.0 with a flow rate of 2.0 BV/h to the column, the eluent was 40 % ethanol, and the flow rate was 1.5 BV/h. After purified by ADS-8, the product purity was 82.7 % with a yield of 66.2 %. Conclusion ADS-8 can be used in purification of total flavonoids of Prunella.