肝硬化患者外周血CXCR1、CXCR2表达及其与HBV载量相关性

Expression of CXCR1,CXCR2 and the correlation to loads of HBV in the peripheral blood of liver cirrhosis

目的:探讨肝硬化患者外周血CXCR1、CXCR2表达及其与HBV载量相关性。方法:以定量PCR检测65例肝硬化患者(代偿期36例,失代偿期29例)外周血CXCR1、CXCR2 mRNA水平,设GAPDH为参照,以logcDNA/logGAPDH比值代表其最终mRNA水平。以Real-time PCR检测患者PBMCs及血清中HBV载量。结果:肝硬化患者PBMCs内CXCR1 mRNA表达量分别为(0.692 5±0.135 4)logcDNA/logGAPDH,较正常组显著增高,差异有显著性(P<0.01),而CXCR2 mRNA表达量为(0.434 4±0.069 4)logcDNA/logGAPDH,较正常组略有下降,但差异无统计学意义(P>0.05)。其中,失代偿组CXCR1mRNA为(0.766 2±0.129 2)logcDNA/logGAPDH,较代偿组升高,差异有显著性(P<0.01),而两组CXCR2表达水平相近,差异无显著性(P>0.05)。肝硬化病毒复制组CXCR1 mRNA水平为(0.749 8±0.110 6)logcDNA/logGAPDH,与HBV载量相关系数为0.380,较非复制组显著升高,差异有统计学意义(P<0.01);CXCR2 mRNA表达量为(0.451 9±0.059 5)logcDNA/logGAPDH,与HBV载量相关系数为0.198,虽较非复制组升高,但差异无统计学意义(P>0.05)。结论:肝硬化患者外周血CXCR1表达明显升高,并与HBV病毒载量呈正相关,CXCR1在参与肝硬化患者因HBV介导的致炎分子病理过程中起重要作用,而CXCR2在介导肝硬化致炎病理过程中作用较弱。

Objective：To investigate the levels of CXCR1, CXCR2 and the correlation to loads of HBV in the peripheral blood of liver cirrhosis. Methods： The quantitative PCR was used to examine the mRNA expression of CXCR1 and CXCR2 in the peripheral blood of sixty-five cirrhosis （ compensated cirrhosis 36, deeompensated cirrhosis 29）. The ratios of logcDNA/logGAPDH were regarded as the extreme levels of CXCRI and CXCR2 mRNA, The loads of HBV in PBMCs and serum were detected by Real-time PCR. Re- sults： The mRNA of CXCR1 in the PBMCs （0. 692 5 ± 0. 135 4 logcDNA/logGAPDH） was significantly higher than that in controls （ P 〈 0.01 ）. The CXCR2 mRNA expression in cirrhosis （0. 434 4 ± 0. 069 4 logcDNA/logGAPDH） was lower than that in normal con- trols, but there was no significant difference between the two groups （ P 〉 0.05 ）. The levels of CXCR1 mRNA in decompensated cir- rhosis was （0. 766 2 ±0. 129 2） logeDNA/logGAPDH, and was significantly higher than that in compensated （ P 〈 0.01 ）. However, the similar expression of CXCR2 mRNA was explored between the two groups （ P 〉 0.05）. The levels of CXCRI mRNA was signifl- eantly higher in positive of HBV-DNA （0. 749 8 ± 0.110 6 logcDNA/IogGAPDH） than that in negative of HBV-DNA （ P 〈 0. 01 ）. The CXCR2 mRNA expression in positive of HBV-DNA （0. 451 9 ± 0. 059 5 logcDNA/logGAPDH） was higher than that in negative of HBV-DNA, but there was few significant differences between the two groups （ P 〉 0.05 ）. The CXCR1 mRNA expression was positively correlated with the loads of HBV-DNA （ r =0. 380, P 〈 0.05 ） in cirrhosis. However, the CXCR2 mRNA expression was negatively correlated with the loads of HBV-DNA （r = 0. 198, P 〉 0.05 ）. Conclusion： The high levels of CXCR1 expression are confirmed in cirrhosis and have positive correlation to loads of HBV, and may play a key role in the inflammatory pathological process caused by HBV in cirrhosis. However, the weaken effects of CXCR2 are established in mediating pathological inflammation in cirrhosis.