不同细胞因子及相关抗体检测在不孕症诊断中的意义

The significance of detection the different cytokines and antibody in patients with infertility

目的:探讨不同细胞因子及相关抗体在不孕症诊断中的作用,为免疫性不孕症的诊治提供新的思路。方法:用ELISA法检测30例免疫性不孕症患者和20例健康女性的血清中IL-2、TNF-α、IFN-γ、IL-4、IL-10、IL-21水平及AhCGAb、AsAb、AOAb、EMAb。结果:不孕症组血清中IL-2、TNF-α、IFN-γ、IL-21含量明显高于对照组(P<0.01),而IL-4、IL-10则无明显变化;AhCGAb、AsAb、AOAb、EMAb的阳性率均明显高于对照组(P<0.01)。结论:Th1型细胞因子含量增高及各种抗体的表达在不孕症发生过程中起重要的作用;结合临床,检测IL-21和T fh细胞可能成为诊断不孕症的新指标。

Objective：To explore the effects of different cytokines and antibody in patients with infertility. Methods： The ex- pression levels of IL-2, TNF-α, IFN-γ, IL-4, IL-10, IL-21 and AhCGAb, AsAb, AOAb, EMAb from 30 women with infertility and 20 healthy women were detected by enzyme-linked immunosorbent assay. Results： The expression levels of IL-2, TNF-α and IFN-γ and IL-21 in infertile women were significantly higher than that in healthy women （P 〈0.01 ）, the expression levels of IL-4 and IL-10 were not marked change ; the positive rates of antibody in infertile women were significantly higher than that in healthy women （ P 〈 0. 01 ） . Conclusion： The high expression of Thl type cytokines and antibody play an important role in the onset of infertility; detec- ting the IL-21 and T fh to become the new index of diagnosis infertility.