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宫颈HPV感染分子生物学检测技术的比较性研究进展 被引量：4

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摘要 已知HPV病毒型别众多且易发生高频突变，故临床检验很难做到完全、准确的检出．现已上市的多种检测HPV的方法，优劣各异，而目前唯有分子生物学检测法获得了国内外临床使用许可。但分子生物学检测技术本身又具有一些问题，如引物的结合区段、检测通量及检测成本等，这些问题使检测设计难以顾全，同时它也没能解决检测HPV型别全覆盖和型别绝对特异等问题。下面就HPV分子生物学检测方法的研究进展进行综述。
作者 付冰冰 肖长义
机构地区 三峡大学医学院
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2013年第10期1106-1110,共5页 Chinese Journal of Immunology
基金 湖北省自然科学基金(2011CDC002)项目资助
关键词 分子生物学检测技术 宫颈HPV感染 HPV型别 生物学检测法 二代杂交捕获 HPV病毒 检测试剂盒 临床检验
分类号 R392 [医药卫生—免疫学]
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参考文献16

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同被引文献43

  • 1郑晓霞,李琼珍,李玲,胥琳璟.宫颈癌预后状况及其影响因素分析[J].实用癌症杂志,2014,29(3):314-316. 被引量：56
  • 2孟庆更,蔡茹艺.HPV16、HPV18感染在宫颈上皮内瘤样病变及宫颈癌中的检测分析[J].北京医学,2007,29(11):664-666. 被引量：3
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  • 5Wang J,Sampath A,Raychaudhuri P,et al.Both Rb and E7 are regulated by the ubiquitin proteasome pathway in HPVcontaining cervical tumor cells[J].Oncogene,2001,20(34):4740-4749.
  • 6Incassati A,Patel D,McCance DJ.Induction of tetraploidy through loss ofp53 and upregulation of Plk1 by human papillomavirus type-16 E6[J].Oncogene,2006,25(17):2444-2451.
  • 7Zheng ZM,Baker CC.Papillomavirus genome structure,expression,and post-transcriptional regulation[J].Front Biosci,2006,11:2286-2302.
  • 8Klaes R,Woerner SM,Ridder R,et al.Detection of highrisk cervical intraepithelial neoplasia and cervical cancer by amplification of transcripts derived from integrated papillomavirus oncogenes[J].Cancer Res,1999,59(24):6132-6136.
  • 9Frohman MA,Dush MK,Martin GR.Rapid production of full-length cDNAs from rare transcripts:amplification using a single gene-specific oligonucleotide primer[J].Proc Natl Acad Sci USA,1988,85(23):8998-9002.
  • 10Ziegert C,Wentzensen N,Vinokurova S,et al.A comprehensive analysis of HPV integration loci in anogenital lesions combining transcript and genome-based amplification techniques[J].Oncogene,2003,22(25):3977-3984.

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中国免疫学杂志
2013年 第10期

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