双靶向TRAIL过表达联合辐射对乳腺癌MDA-MB-435细胞增殖和凋亡的影响

The effects of dual targeting TRAIL overexpression combined with radiation on proliferation and apoptosis in breast cancer MDA-MB-435 cells

目的探讨Egr-1和hTERT双靶向介导TRAIL过表达联合辐射对乳腺癌细胞增殖和凋亡的作用,为肿瘤基因-放射治疗提供必要的参考。方法实验分为正常对照组(Control)、CRAd.pEgr-1-TRAIL组、2.0Gy照射组及CRAd.pEgr-1-TRAIL+2.0Gy照射组。病毒感染和X射线照射后,分别采用CCK-8试剂盒和AnnexinⅤ-FITC试剂盒检测细胞增殖和细胞凋亡。结果随着时间延长,各组细胞均表现增殖状态,control和CRAd.pEgr-1-TRAIL组MDA-MB-435细胞增殖较快,且无明显差异,而2Gy和CRAd.pEgr-1-TRAIL+2.0Gy组细胞增殖较慢,且以CRAd.pEgr-1-TRAIL+2.0Gy组更明显,48h时甚至出现增殖抑制;2.0Gy组(24和48h)和CRAd.pEgr-1-TRAIL+2.0Gy组(12,24和48h)均较control组显著增加(P<0.05,P<0.01),而且CRAd.pEgr-1-TRAIL+2.0Gy组(24和48h)也较2.0Gy组显著增加(P<0.05,P<0.01)。与control组比较,CRAd.pEgr-1-TRAIL组细胞凋亡无显著变化,而2.0Gy组和CRAd.pEgr-1-TRAIL+2.0Gy组细胞凋亡显著增加(P<0.05,P<0.01),尤其以CRAd.pEgr-1-TRAIL+2.0Gy组更明显,与2.0Gy组比较,具有统计学差异(P<0.05)。结论电离辐射能诱导MDA-MB-435细胞增殖抑制和凋亡增加,双靶向介导的TRAIL过表达能增强这种作用。

Objective To explore the effects of dual targeting TRAIL overexpression combined with radiation on proliferation and apoptosis in breast cancer MDA-MB-435 cells,and provide necessary reference for tumor gene-radio- therapy. Methods There were control,CRAd, pEgr-I-TRAIL,2.0 Gy and CRAd. pEgr-I-TRAIL＋2.0 Gy groups in the experiment. After infection with adenovirus and irradiation with X-rays, proliferation and apoptosis were measured- with CCK-8 and Annexin V-FITC kits, respectively. Results With time prolongation, MDA-MB-435 cells in each groups all showed proliferative state, their proliferation in control and CRAd. pEgr-TRAIL groups was very fast, and had no differenc between them;but the proliferation in 2.0 Gy and CRAd. pEgr-TRAIL＋ 2.0 Gy groups was slower, and it was more obvious in CRAd. pEgr-TRAIL＋ 2.0 Gy group, even there was inhibitory phenomenon at 48 h; compared with control group,the proliferation in 2 Gy group （24 and 48 h） and CRAd. pEgr-I-TRAIL ＋2.0 Gy group （12,24,and 48 h） increased significantly （P〈0.05,P〈0.01） ,and that in CRAd. pEgr-I-TRAIL ＋2.0 Gy was more obvious than that in 2.0 Gy group （P〈0.01）. As compared with control,the apoptotic ceil percentages in CRAd. pEgr- I-TRAIL group had no significant change, but those in 2.0 Gy and CRAd. pEgr-I-TRAIL ＋2. 0 Gy groups all in- creased significantly （P〈0.05, P〈0.01 ）, especially in CRAd. pEgr-I-TRAIL ＋ 2.0 Gy group, those were more obvi- ous than those in 2.0 Gy group（P〈0.05）. Conclusion Ionizing radiation could induce MDA-MB-435 cell proliferation inhibition and apoptosis increasing,dual targeting TRAIL overexpression could enhance the effects.