绞股蓝总皂苷对大鼠脑缺血再灌注损伤的保护作用

Therapeutic effects of Gypenosides on cerebral ischemia Reperfusion injury in rats

目的探讨绞股蓝总皂苷(Gypenosides,GP)对大鼠脑缺血再灌注损伤的保护作用及其机制。方法采用线栓法建立大鼠脑缺血再灌注损伤模型,实验分为假手术组、模型组、血塞通阳性药组(20mg·kg-1)、绞股蓝总皂苷高、中、低剂量组(100、30和10mg·kg-1),观察绞股蓝总皂苷对脑缺血再灌注大鼠神经功能评分、脑组织匀浆中SOD、MDA、NO含量等生化指标及梗死面积变化的影响,同时观察HE病理改变,用免疫组化方法观察绞股蓝总皂苷对Bcl-2、Bax蛋白表达的影响。结果与模型组比较绞股蓝总皂苷可改善大鼠神经功能评分(P<0.05),提高大鼠脑组织中SOD活性(P<0.05),降低其MDA含量(P<0.05),缩小脑梗死面积(P<0.05)。免疫组化结果显示绞股蓝总皂苷可明显提高Bcl-2呈阳性的细胞数(P<0.05),降低Bax阳性的细胞数(P<0.05)。结论绞股蓝总皂苷能够改善大鼠脑缺血再灌注损伤,可通过保护脑组织抗氧化酶的活性,抑制脂质过氧化反应,减轻自由基对脑组织的损害,对缺血再灌注脑组织具有治疗作用。

Objective To investigate the effect of Gypenosides （GP）on the injury induced by cerebral ischemia reperfusion in rats and its protective mechanisms. Methods In this study,we set up cerebral ischemia reperfusion mod- el in rats by blocking middle cerebral artery. Rats were randomly divided into six groups：sham group, model group, Shuxuening group and GP-treated groups（100,30 - 10 mg·kg^-1 ）. To observe the neurologic deficit score,the level of SOD, MDA, NO,infarction area in the brain tissue and HE Histopathological change. Immunohistochemistry method was used to measure Bcl-2 and Bax expressions. Results Compared with model group,GP groups could significantly reduce the neurologic deficit score,increase the SOD activity, decrease the MDA contents and infarction area of the brain tissue. The Immunohistochemistry results showed that GP could significantly increase the expression of Bcl-2 and re- duce the expression. Conclusion GP could protect the cerebral ischemia reperfusion in rats and the mechanisms may be related to its anti-oxidation activity of the enzyme, enhance the ability of removing free radicals and reduce the free radi- cal from ischemia-reperfusion