气管滴注纳米二氧化硅颗粒致大鼠肺炎症反应及脏器中硅浓度变化的研究

The Study of Pulmonary Inflammatory Reaction and Change of Si Concentration in tissues of Silica Nanoparticles in Rats after Intracheal Instillation

目的对纳米二氧化硅颗粒导致大鼠肺脏的炎症反应进行研究。方法将128只雄性Wistar大鼠随机分为4组,分别为空白对照组(生理盐水)、0.5、1和2mg/Kg·bw剂量组,采用非暴露式气管滴注染毒一次,分别于染毒后的1d、7d、14d以及28d后处死大鼠,每实验组各处死8只动物,分别检测分析脏器中的硅浓度的变化;检测肺泡灌洗液中白细胞总数、蛋白含量、乳酸脱氢酶。结果纳米二氧化硅颗粒主要分布在肝脏和肾脏;可引起大鼠肺部明显的炎症反应;可引起肺泡灌洗液中的白细胞总数、蛋白含量、乳酸脱氢酶升高。结论纳米二氧化硅颗粒可引起肺组织损伤,对大鼠具有一定的肺毒性作用。

Objective To study the pulmonary inflammatory reaction of silica nanoparticles in rat after intracheal In- stillation. Methods 128 Wistar rats divided into 4 groups at random and exposed to blank control and different doses of exposed groups （0.5,1.0, and 2.0 mg/Kg ·bw） by intra-tracheal instillation only once. And rats were neerospied at 1 day, 7 day, 14 day, and 28 day after exposure. Si concentration of tissues for rats was detected. The bronchoalveolar lav- age fluid （BALF） was collected and analyzed; Total white cell count, total protein （TP）, and lactic dehydrogenase （LDH） in lung BALF were measured. Results Silica nanoparticles are mainly distributed in the liver and kidneys; can induce significant lung inflammation,and cause total white cell count,TP, and LDH in BALF increased. Conclusion Silica nanoparticles can cause lung tissue damage in rats with a certain lung toxicity.