有氧运动对糖尿病大鼠主动脉胶原代谢的影响及其可能机制研究

Impact and metabolism of exercise on collagen deposition in artery of diabetic rats

目的:探讨长期有氧运动对糖尿病大鼠主动脉晚期糖基化终产物形成及其与胶原代谢的关系。方法:通过高脂喂养加腹腔注射小剂量链脲佐菌素(STZ)构建2型糖尿病SD大鼠模型。将SD大鼠分为正常对照组(C)、糖尿病模型组(D)和糖尿病运动组(DE)。糖尿病运动组(DE)大鼠进行不负重游泳运动12周。实验结束后检测大鼠血液GLU、GHb、GSP、Insulin、TG、TC、HDL-C、LDL-C和主动脉AGEs、Hyp、SOD、MDA及Col I、Col IV、FN表达的变化,并对实验结果进行统计分析。结果:与正常对照组(C)相比,糖尿病模型组(D)的血液GLU、GHb、GSP、Insulin、TG、TC、LDL-C水平显著升高(P<0.05或P<0.01),主动脉AGEs、Hyp、MDA含量和Col I、Col IV、FN表达水平均显著升高(P<0.01),血液HDL-C和主动脉SOD水平显著降低(P<0.01);与糖尿病模型组(D)相比,糖尿病运动组(DE)的血液GLU、GHb、GSP、TG、TC、LDL-C水平显著降低(P<0.01),主动脉AGEs、Hyp、MDA含量和Col I、Col IV、FN表达显著降低(P<0.01),SOD显著升高(P<0.01)。结论:长期有氧运动可改善2型糖尿病大鼠主动脉间质胶原沉积的现象,这可能与运动降低血糖、主动脉AGEs含量和氧化应激水平有关。

In order to investigate the impact and metabohsm oi exercise on collagen deposition in artery of diabetic rats, the authors established the model for type 2 diabetes by high - fat diet - fed and low - dose streptozotocin - treated. The diabetic rats were randomly divided into type 2 diabetes control group （D） , type 2 diabetes exercise group （DE） , and normal control group （C）. The DE group swam without load for 12 weeks. At the end of the experiment, the levels of GLU, GHb, GSP, Insulin, TG, TC, HDL - C, LDL - C in blood and the levels of AGEs, Hyp, SOD, MDA, Col I,Col IV, FN in artery were tested. The experimental results revealed the following findings： The levels of blood GLU, GHb, GSP, Insulin, TG, TC, LDL - C and artery AGEs, Hyp, Col l, Col IV, FN, oxidative stress were significantly higher（ P 〈 0.05 or P 〈 0.01 ） while SOD, HDL - C were significantly lower in group D than those in group C （P 〈0.01 ） ; The levels of blood GLU, GHb, GSP, TG, TC, LDL- C and artery AGEs, Hyp, Col I, Col IV, FN, oxidative stress were significantly lower in group DE than those in group D （ P 〈 0.01 ）. The results indicate that exercise can improve arterial collagen deposition of type 2 diabetic rats, which may be related with the reduction of blood GLU and arterial AGEs, oxidative stress of type 2 diabetic rats.