急性心肌梗塞患者血小板蛋白激酶C高反应活性的研究

A Study on the Role of Platelets Protein Kinase C in Acute Myocardial Infarction

目的 :研究血小板蛋白激酶 C(PKC)在急性心肌梗塞 (AMI)中的活性 ,以揭示 PKC的致病机制。 方法 :利用 P-磷酸二氢钠标记血小板 ,以 PKC的底物 40 KD蛋白的磷酸化为酶激活的标志 ,对 AMI组及对照组PKC的基础活性及反应活性进行检测。经 t检验进行分析比较 ,同时检测两组的血小板数及血小板膜表面 α颗粒膜蛋白140 (GMP- 140 )分子数。 结果 :AMI组 PKC基础活性及经凝血酶刺激后的反应活性均较对照组显著增高 (P<0 .0 5 ) ;血小板膜表面 GMP-140水平较对照组显著增高 (P<0 .0 1)。 结论 :AMI中血小板

Objective:To study platelet protein kinase C(PKC) activity and its role in patients with acute myocardial infarction (AMI) Methods:Platelates were labelled with 32 p NaH 2PO 4 and the phosphorylation of platlet membrane 20KD 40KD substrate was considered the mark of activated PKC was.The baseline and responsive activity of platelet PKC between.AMI group and control group were compared and analysed. Results:The baseline activity and responsive activity of platelet PKC after thrombin′s stimulation in patient group were remarkably elevated than that in control group ( p <0 05, p <0 01 respeetively).The GMP 140 level on platelet surface in patient group were remarkably elevated than that in control group ( p <0 01) Conclusion:The unusual activated PKC and its high responsiveness played important roles in platelet thrombosis for the patients with AMI.