摘要
目的:通过观察祛痰活血汤对非酒精性脂肪肝模型大鼠肝功、血脂等血液生化指标、肝组织病理形态学改变以及肝细胞核转录因子KB(NF—v,B)表达的影响,探讨其对非酒精性脂肪肝(nonalcoholicfattyliverdisease,NAFLD)的干预及作用机制。方法:取健康sD大鼠60只,雄性,适应性喂养1周,随机分为2组,正常对照组(A组)及造模组,其中正常组10只,普通饲料喂养;造模组50只,高脂饲料喂养。造模组经过高脂饲料喂养2周后,将其再次分组并开始按大鼠的体重进行灌胃给药。造模组50只大鼠分为模型对照组(B组)、祛痰活血汤低剂量治疗组(L组)、祛痰活血汤中剂量治疗组(M组)、祛痰活血汤高剂量治疗组(H组)、阳性对照组(凯西莱组)共5组,每组10只。B组、A组不用任何药物,灌胃10ml/kg的生理盐水。各组实验动物自由进食,每2周称重,并根据情况调整药品干预剂量。造模组在分组灌胃给药的第8、9、10周周末,随机抽取1~2只大鼠处死,抽血观察生化指标(天门冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、甘油三酯(TG)、总胆固醇(TC)等)以及肝组织病理学变化。第10周末处死所有大鼠,检测肝功能、血脂等,并取肝脏组织制备石蜡切片,观察肝组织病理学变化以及检测肝细胞NF—I(B表达的情况。结果:与正常对照组比较,模型对照组大鼠血清肝功能和血脂指标,肝脏病理炎症活动度、肝细胞NF—td3的表达水平均有显著的升高、加重,差异具有统计学意义(P〈0.01);祛痰活血汤三个剂量组、阳性对照组与模型对照组比,各项指标均有不同程度的减轻或者降低,结果具有统计学意义(P〈O.05)。结论:祛痰活血汤能有效逆转非酒精性脂肪肝大鼠肝组织脂肪变性程度,改善肝功能、血脂等血液生化指标,具有调节脂质代谢紊乱,降低肝组织NF-Id~表达的作用,这可能是其防治NAFLD的作用机制之一。
Objective: To investigate the influence of QutanHuoxue decoction on rat model of non-alcoholic fatty liver disease(NAFLD) and its possible mechanism. Methods: 60 healthy male SD rats were randomly divid- ed into normal control group (group A), model control group (group B), Qutan Huoxue decoction low dose group (group L), medium dose group (group M), high dose group (H group), and positive control tiopronin group (group K). At the end of 10 week all rats were sacrificed, the liver function (AST, ALT), blood lipid(TG,TC) were tested, and paraffin sections of liver tissue were made to observe pathological changes and the expression of NF- KB. Results: Compared with group A, serum lipid levels and liver function, liver pathologic inflammatory activity, hepatocyte NF-KB expression index in group B were significantly increased (P 〈 0.01 ); compared with group B, all the above indicators in group L,M,H and K were significantly reduced or mitigated (P 〈 0.05). Conclusion:Qutan Huoxue Decoction can significantly reduce liver steatosis and blood biochemial indices, im- prove liver function, regulate lipid metabolism, and reduce the expression of NF-KB in liver tissue, and the latter may be the mechanism of prevention of non-alcoholic fatty liver.
出处
《泸州医学院学报》
2013年第5期441-446,共6页
Journal of Luzhou Medical College
基金
"十一五"国家中医药管理局"名老中医传承工作室建设项目"[(国中医药人教发[2010]59号)
四川省教育厅重点项目[10ZA154(川教函[2010]621号)
泸州市科技局重点科技项目[2010-s-15(1/8)(泸市财企[2010]41号)]