冷冻载体Cryotop降温速率的测量及系统优化

Cooling Rate Measurement and System Optimization for Cryotop Device

Cryotop法是玻璃化保存卵母细胞较有效的方法之一。当Cryotop载体插入液氮时,降温速度极高,几乎瞬间完成,常规的方法无法测量。实验使用数字示波器、线径25μm的T型热电偶建立起高速测温系统,测量了Cryotop法用于卵母细胞低温保存时的降温速率。通过改变载体材料、厚度、冷源温度等条件,提高Cryotop的降温速率。结果发现当使用60μm的铜质载板,使用浆状液氮作为冷源时,降温速度最高,可达到(37130±1336)K/min,较商品化的Cryotop的降温速度((11982±1936)K/min)提高了2倍。

Cryotop is one of the best effective methods for ooeytes cryopreservation. When Cryotop is inserted into liquid nitrogen, the cooling speed is extremely high, which is difficult to measure by conventional method. In this study, digital oscilloscopes and type T thermocouple of 25 .王海松.m diameter were employed to establish a high speed temperature measurement system. The cooling rates of Cryotop device at different cartier material, thickness and cold source temperature were measured. The results showed that when 60.王海松.m copper was used as carrier and slush nitrogen was used as cold source, the cooling rate was increased. It could reach （37130 ± 1336）K/min, which was twice higher than that of commercially available Cryotop （ （11982 ± 1936）K/min）.