两种途径提高红麻不定芽诱导率的研究

Differentiation Induction of Kenaf Adventitious Buds through Two Approaches

为了提高红麻愈伤组织不定芽诱导率,以红麻子叶和胚轴为外植体,采用两种途径诱导红麻不定芽分化,对影响红麻不定芽分化的培养基组成因素进行研究。结果表明:(1)在诱导愈伤组织分化不定芽的途径中,两种外植体均可诱导产生愈伤组织,但是胚轴产生的愈伤组织质量优于子叶。在分化培养基MS+6-BA(1mg/L)+2,4-D(1mg/L)+GA3(1mg/L)中,愈伤组织可分化少量不定芽,但是分化率较低;当该分化培养基中添加0.3%的活性炭时,愈伤组织在分化不定芽的同时也产生了大量不定根。(2)在通过直接诱导外植体分化不定芽的途径中,胚轴难以诱导产生不定芽,子叶可有效诱导不定芽;在MS+TDZ(0.1mg/L)+NAA(0.1mg/L)的诱导培养基中,子叶的不定芽诱导效果最好,诱导率为64.29%。而在诱导培养基中添加AgNO3后,不定芽的诱导率明显提高,其诱导效果最好的培养基为MS+TDZ(0.1mg/L)+NAA(0.1mg/L)+AgNO3(1mg/L),此时不定芽诱导率可达85%。

Cotyledon and hypocotyl were used as explants to study the influential factors of kenaf ad -ventitious buds differentiation through two approaches.The results showed that：（ 1 ） in the approach of callus induction , the quality of hypocotyl callus was better than cotyledon , callus could be induced to dif-ferentiate a few adventitious buds in the medium of MS ＋6-BA （1mg/L） ＋2, 4-D （1mg/L） ＋GA3 （1mg/L）, but the induction rate was very low.After adding 0.3%activated carbon in the medi-um, many roots were induced along with adventitious buds.（2） In the approach of inducing explants di-rectly, hypocotyl hardly generated adventitious buds , cotyledon could induce adventitious effectively.Cotyledon could get the highest induction rate in the medium of MS ＋TDZ （ 0.1 mg/L ） ＋NAA （0.1mg/L）, and the rate was 64.29%.After adding AgNO3 in the medium, the rate could increase ob-viously.The best induction medium was MS ＋TDZ （0.1mg/L） ＋NAA （0.1mg/L） ＋AgNO3 （1mg/L） , and the adventitious buds induction rate was 85%.