期刊文献+

1986--2011年广西猪瘟流行监测及遗传变异分析 被引量:7

Epidemic surveillance and genetic variation analysis of swine fever virus in Guangxi in the past 25 years
原文传递
导出
摘要 采用Nested-PCR方法对采自广西各地1986-2011年间,199个猪场(养殖户)的1 521份样品进行CSFV、PRRSV、PCV2、PRV、HPS等病原的检测及流行病学调查,结果发现广西猪瘟以混合感染为主,病性复杂。尽管保育猪发病仍占第1位,但近年来母猪繁殖障碍、无症状带毒感染的发病率已明显提高。通过对25年来收集的77株广西CSFV流行株E2基因的比较分析,发现广西猪瘟流行株分为基因I群和II群,目前仍未发现基因III群。起源于欧洲大陆的S2.1亚型毒株已经成为广西的优势流行株,多引起临床的急性和亚急性感染;以SHIMEN株为代表的中国经典毒株S1.1亚型是造成母猪繁殖障碍、隐性带毒感染的主要毒株。流行于20世纪80-90年代间的基因S2.2及S2.3亚型毒株已经基本消失,值得进一步研究。广西CSFV流行株E2蛋白部分关键氨基酸位点已经发生变异,可能会导致疫苗的不完全保护。 One thousand five hundred and twenty one samples from 199 farms (farmers) in Guan gxi in the last 25 years (19862011)were detected by NestedPCR method for detection and the epidemiological survey of as CSFV, PRRSV, PCV2, PRV, HPS. The results showed that mixed infec tion was dominant with the CSF in Guangxi, showing a complexity in type of the disease. Although incidence in weaned piglets still accounted for the first place,a significant boost was shown in re productive failure and asymptomatic infection with virus in sows,in the resent years. Comparative analysis of 77 CSFV Guangxi epidemic strains of E2 gene collected in the last 25 years demonstra ted that the currently popular CSFV in Guangxi contains gene group I and group II,whereas gene group III has not been perceived so far. But the S2.1 subtype originated in continental Europe has become dominant epidemic strains in Guangxi, generally causing acute and subcute clinical infec tion. The native typical gene SHIMEN strains of S1.1 subtype remains epidemic in Guangxi pigs, being the main strains in causing reproductive failure in sows and hidden infection with virus; gene S2.2 and S2.3 subtype prevailing during the 1980s and 1990s have disappeared, the causes of which de serve further study. The key E2 protein amino acid sites of CSFV Guangxi epidemic strains have mutated, which may lead to unsound protection of the vaccine.
出处 《中国兽医学报》 CAS CSCD 北大核心 2013年第10期1481-1487,共7页 Chinese Journal of Veterinary Science
基金 科技部成果转化资金资助项目(2009GB2E10029) 农业部农业科技跨越计划资助项目(2009跨21) 广西公益科研院所基本科研业务专项(桂科专项10-4) 广西科技攻关与新产品试制资助项目(桂科攻1123007-3)
关键词 CSFV E2 Nested—PCR 序列分析 E2 gene of CSFV Nested PCR sequence analysis
  • 相关文献

参考文献8

  • 1姚文生,范学政,王琴,宁宜宝.我国猪瘟流行现状与防控措施建议[J].中国兽药杂志,2011,45(9):44-47. 被引量:75
  • 2Van Rijn P A,Van Gennip H G,De Meijer E J,et al. Epitope mapping of envelope glyeoprotein E1 of hog- cholera virus strain Brescia [J. J Gene Virol, 1993, 74:2053-2066.
  • 3王仕荣,向晖,吴健敏,王仰杰,梁安莉,覃绍敏,黄红梅,陈凤莲,马玲,谢彬,邵帅,袁书智.广西“猪高热综合征”主要病原调查及PRRSV Nsp2基因变异分析[J].中国兽医学报,2009,29(3):249-253. 被引量:17
  • 4Paton D, MeGoldriek A. Genetieing of classical swine fevervirusJ]. Vet Microbiol, 2000,73 : 137-157.
  • 5李丛丛,何成强,张明华,李睿坤.猪瘟病毒同源重组的试验研究[J].现代农业科技,2008(17):259-260. 被引量:1
  • 6He C Q,Ding N Z,Chen J G,et al. Evidence of natural recombination in classical swine fever virus[J. Virus Res,2007,126 (1/2) :179-185.
  • 7Van Rijn P A,Rene G P,van Genni P,et al. A Prelim- inary map of epitopes on envelope glyeoprotein E1 of HCV strain Breseia[J. Vet Microbiol, 1992,33 221- 230.
  • 8Van Rijn P A, van GeniP H G P, Meijer E J, et al. Epitope maping of envelope glyeoprotein E1 of hog eholera virus strain BreseiaJ. Gene Virol, 1993,741 2053 2060.

二级参考文献32

共引文献90

同被引文献81

  • 1蔡宝祥.猪瘟的免疫综述[J].动物科学与动物医学,2005,22(2):24-27. 被引量:17
  • 2金颜辉.2004~2005年福建省部分猪场主要疫病流行病学调查及检测报告[J].福建畜牧兽医,2006,28(3):5-6. 被引量:11
  • 3Luo Y, Li S, Sun Y, et al. Classical swine fever in China:a minireviewfj]. Vet Microbiol, 2014,172( 1/2): 1-6.
  • 4Paton DJ, McGoldrick A, Greiser-Wilke I, et al. Genetictyping of classical swine fever virus. Vet Microbiol, 2000,73(2/3):137-157.
  • 5Chen N,Hu H, Zhang Z,et al. Genetic diversity of theenvelope glycoprotein E2 of classical swine fever virus: recentisolates branched away from historical and vaccine s trains [J],Vet Microbiol, 2008,127(3/4): 286-299.
  • 6Sun SQ, Yin SH, Guo HC, et al. Genetic typing of classicalswine fever virus isolates from China[J]. Transbound EmergDis,2013,60(4):370-375.
  • 7MEYERS G,THlEL HJ.Molecular characterization of pestivirus[J].Adv-virus Res,1996,47:53-118.
  • 8MOORMANN RJ,VAN Gennip HG,MIEDEMA GK,et al.Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus[J].J Virol,1996,70:763-770.
  • 9RUGGLI N,TRATSCHIN JD,MITTELHOLZER C,et al.Nucleotide sequence of classic swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA[J].J Virol,1996,70:3478-3487.
  • 10LOWINGS P,IBATA G,NEEDHAM G,et al.Classic swine fever diversity and evolution[J].Gen Virol,1996,77(6):1311-1321.

引证文献7

二级引证文献57

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部