2类阳离子表面活性剂对细胞的增殖抑制作用

Study of proliferation inhibiting effect of two kinds of cationic surfactants on cells

通过噻唑蓝(MTT)比色法检测了4种单长链烷基三甲基氯化铵和3种单长链烷基二甲基苄基氯化铵系列阳离子表面活性剂对人宫颈癌细胞(HeLa)及中华仓鼠卵巢细胞(CHO)的增殖抑制作用,探讨了2类结构相似物质对细胞毒性的大小。结果表明:单长链烷基三甲基氯化铵和单长链烷基二甲基苄基氯化铵对细胞的增殖抑制作用均随其浓度的增加而增强;相同浓度下,对细胞的增殖抑制作用均随其脂肪链长度的增加而增强;单长链烷基三甲基氯化铵的细胞毒性小于相同脂肪链长度的单长链烷基二甲基苄基氯化铵。

MTT assay is adopted to study of proliferation inhibiting effect of four kinds of alkyl trimethyl ammonium chlorides and three kinds of alkyl dimethyl henzyl ammonium chlorides on HeLa cells and CHO cells, and to discuss cyto-toxicity of two kinds of cationic surfaetants which are structurally related. The results indicated that the proliferation inhibiting effect of alkyl trimethyl ammonium chlorides and alkyl dimethyl henzyl ammonium chlorides on both HeLa cells and CHO cells gradually increased with concentration increasing; and as for the same concentration, proliferation inhibiting effect of both two kinds increased with the number of carbons increasing. Cytotoxicity of alkyls dimethyl benzyl ammonium chloride is stronger than that alkyls trimethyl ammonium chloride.